ASSESSMENT OF NITRITE LEVEL - NITRIC OXIDE DERIVATIVE - IN HORSES WITH INTESTINAL COLIC BY ESR SPECTROSCOPY

Abstract

The pathogenesis of diseases of the digestive tract of horses, accompanied by the development of inflammation and oxidative stress, can be associated with a lack of nitrogen monoxide, which controls a variety of signaling pathways in the body. Nitrogen monoxide (NO) takes part in the work of the immune and nervous systems, its level directly determines the tone of all blood vessels and the course of many pathological processes. NO should be considered as one of the most important factors in the protection of the gastric and intestinal mucosa. The article describes in detail the method for determining the level of nitrite-nitric oxide metabolite - in diseases of horses with the symptom complex of “true” colic, and also presents the results of measurements of nitrite in the serum of healthy animals of two age groups: 1-5 years and 6-25 years and animals with pathology. The concentration of nitrite in blood serum in horses of the age group of 6-25 years was 3.42 ± 4.22 μM, while in young animals (1-5 years) the level of this indicator was 8.24 ± 5.42 μM, which is 2,4 times higher. A sharp decrease of nitrite was noted in all horses with intestinal diseases of 2.07 ± 0.9 μM, especially with meteorism 0.6 ± 0.4 μM and spastic colic 1.78 ± 0.5 μM. These facts determine the diagnostic and prognostic value of nitric oxide as a biomarker for the regulation of intestinal motility in normal and pathological conditions.

Keywords

• horse
• intestinal colic
• ESR-spectroscopy
• nitrite
• nitric oxide
• nitrosothioles
• dinitrosyl
• iron complexes
• nitrosyl hemoglobin