Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I

Sarah E. Lang; Tamara K. Stevenson; Tabea M. Schatz; Brandon J. Biesiadecki; Margaret V. Westfall

Data in Brief (Dec 2017)

Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I

Sarah E. Lang,
Tamara K. Stevenson,
Tabea M. Schatz,
Brandon J. Biesiadecki,
Margaret V. Westfall

Affiliations

Sarah E. Lang: Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI 48109, USA; Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI 48109, USA
Tamara K. Stevenson: Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI 48109, USA
Tabea M. Schatz: Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI 48109, USA
Brandon J. Biesiadecki: Department of Physiology and Cell Biology and Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA
Margaret V. Westfall: Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI 48109, USA; Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI 48109, USA; Corresponding author at: Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI 48109, USA.

Journal volume & issue: Vol. 15
pp. 562 – 566

Abstract

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Secondary phosphorylation develops in myocytes expressing phospho-mimetic cardiac troponin I (cTnI) but it is not known whether multiple substitutions (e.g. cTnISDTD and cTnIS4D) cause preferential phosphorylation of the remaining endogenous or the phospho-mimetic cTnI in intact myocytes. Western analysis was performed to determine whether the FLAG/total cTnI ratios are similar for phosphorylated versus total cTnI in myocytes expressing phospho-mimetic cTnI with Asp(D) substitutions at S43/45 plus S23/24 (cTnIS4D) or T144 (cTnISDTD). Representative Western analysis of phosphorylated S23/24 (p-S23/24) and S150 (p-S150) are presented along with re-probes using an antibody which detects all cTnI (MAB1691 Ab). The level of p-S150 also is compared to results obtained using single S43D and/or S45D phospho-mimetic substitutions. These results are discussed in more detail in Lang et al. [1]

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

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