Camellia sinensis Chloroplast Fluoride Efflux Gene CsABCB9 Is Involved in the Fluoride Tolerance Mechanism

Bingbing Luo; Min Guang; Wenjing Yun; Shitao Ding; Suna Ren; Hongjian Gao

doi:10.3390/ijms23147756

International Journal of Molecular Sciences (Jul 2022)

Camellia sinensis Chloroplast Fluoride Efflux Gene CsABCB9 Is Involved in the Fluoride Tolerance Mechanism

Bingbing Luo,
Min Guang,
Wenjing Yun,
Shitao Ding,
Suna Ren,
Hongjian Gao

Affiliations

Bingbing Luo: Anhui Province Key Lab of Farmland Ecological Conservation and Pollution Prevention, Engineering and Technology Research Center of Intelligent Manufacture and Efficient Utilization of Green Phosphorus Fertilizer of Anhui Province, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Min Guang: Anhui Province Key Lab of Farmland Ecological Conservation and Pollution Prevention, Engineering and Technology Research Center of Intelligent Manufacture and Efficient Utilization of Green Phosphorus Fertilizer of Anhui Province, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Wenjing Yun: Anhui Province Key Lab of Farmland Ecological Conservation and Pollution Prevention, Engineering and Technology Research Center of Intelligent Manufacture and Efficient Utilization of Green Phosphorus Fertilizer of Anhui Province, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Shitao Ding: Anhui Province Key Lab of Farmland Ecological Conservation and Pollution Prevention, Engineering and Technology Research Center of Intelligent Manufacture and Efficient Utilization of Green Phosphorus Fertilizer of Anhui Province, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Suna Ren: Anhui Province Key Lab of Farmland Ecological Conservation and Pollution Prevention, Engineering and Technology Research Center of Intelligent Manufacture and Efficient Utilization of Green Phosphorus Fertilizer of Anhui Province, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Hongjian Gao: Anhui Province Key Lab of Farmland Ecological Conservation and Pollution Prevention, Engineering and Technology Research Center of Intelligent Manufacture and Efficient Utilization of Green Phosphorus Fertilizer of Anhui Province, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China

DOI: https://doi.org/10.3390/ijms23147756
Journal volume & issue: Vol. 23, no. 14
p. 7756

Abstract

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Soil is a main source of fluoride for plants. The tea plants (Camellia sinensis) accumulate excessive amounts of fluoride in their leaves compared to other plants, but their fluoride tolerance mechanism is poorly understood. A chloroplast fluoride efflux gene (CsABCB9) was newly discovered by using transcriptome analysis, cloned from Camellia sinensis, and its function was demonstrated in the fluoride detoxication mechanism in Escherichia coli/Xenopus laevis oocytes and Arabidopsis thaliana. CsABCB9 is expressed in tea leaves upon F− treatment. The growth of tea, E. coli, and Arabidopsis were inhibited by F− treatment. However, growth of CsABCB9-overexpression in E. coli was shown to increase with lower fluoride content under F− treatment compared to the control. Furthermore, chlorophyll, xanthophyll and soluble sugar contents of CsABCB9-overexpression in Arabidopsis were improved under F− treatment compared to the wild type. CsABCB9 functions in fluoride transport, and the mechanism by which CsABCB9 improves fluoride resistance in tea is mainly chloroplast protection through fluoride efflux.

Published in International Journal of Molecular Sciences

ISSN: 1661-6596 (Print); 1422-0067 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General); Science: Chemistry
Website: http://www.mdpi.com/journal/ijms

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