Initial Study Of The Method For Detection EBNA-1 In Biopsy Tissues Collected From Nasopharyngeal Carcinoma Patients

Abstract

In Viet Nam, Nasopharyngeal carcinoma (NPC), originating in the nasopharynx, is the most common cancer of the head and neck cancers. The association of NPC with Epstein –Barr virus (EBV) firmly demonstrated that EBV played an important role in nasopharynxcarcinogenesis. For the aim to establish the method for early diagnosis and prognosis of NPC, EBNA-1 (Epstein –Barr nuclear antigen 1) which is necessary for infection of EBV, was chosen as target gene to establish the method for detection of EBV. In this study, we systematically analyzed the mean of percentage weighted of the presence of EBNA-1 in various sources of sample. Moreover, we initially succeed to establish the PCR method for detection of EBNA-1 in biopsy tissues collected from nasopharyngeal cancer patients in Cho Ray hospital. These results indicated that, in the future, it will be useful in developing a non-invasive method for early diagnosis and prognosis of NPC based on molecular techniques in detection of concerning genes which were EBV infection required.

Keywords

• ebv
• ebna-1
• ung thư vòm họng