The Production of Recombinant African Swine Fever Virus Lv17/WB/Rie1 Strains and Their In Vitro and In Vivo Characterizations
Stefano Petrini,
Cecilia Righi,
István Mészáros,
Federica D’Errico,
Vivien Tamás,
Michela Pela,
Ferenc Olasz,
Carmina Gallardo,
Jovita Fernandez-Pinero,
Eszter Göltl,
Tibor Magyar,
Francesco Feliziani,
Zoltán Zádori
Affiliations
Stefano Petrini
National Reference Centre for Pestiviruses and Asfivirus, Istituto Zooprofilattico Sperimentale Umbria-Marche “Togo Rosati”, Via Gaetano Salvemini, 1, 06126 Perugia, Italy
Cecilia Righi
National Reference Centre for Pestiviruses and Asfivirus, Istituto Zooprofilattico Sperimentale Umbria-Marche “Togo Rosati”, Via Gaetano Salvemini, 1, 06126 Perugia, Italy
István Mészáros
HUN-REN Veterinary Medical Research Institute (VMRI), Hungária krt. 21, 1143 Budapest, Hungary
Federica D’Errico
National Reference Centre for Pestiviruses and Asfivirus, Istituto Zooprofilattico Sperimentale Umbria-Marche “Togo Rosati”, Via Gaetano Salvemini, 1, 06126 Perugia, Italy
Vivien Tamás
HUN-REN Veterinary Medical Research Institute (VMRI), Hungária krt. 21, 1143 Budapest, Hungary
Michela Pela
National Reference Centre for Pestiviruses and Asfivirus, Istituto Zooprofilattico Sperimentale Umbria-Marche “Togo Rosati”, Via Gaetano Salvemini, 1, 06126 Perugia, Italy
Ferenc Olasz
HUN-REN Veterinary Medical Research Institute (VMRI), Hungária krt. 21, 1143 Budapest, Hungary
Carmina Gallardo
European Union Reference Laboratory for ASF (EURL-ASF), Centro de Investigación en Sanidad Animal (CISA-INIA, CSIC), Valdeolmos, 28130 Madrid, Spain
Jovita Fernandez-Pinero
European Union Reference Laboratory for ASF (EURL-ASF), Centro de Investigación en Sanidad Animal (CISA-INIA, CSIC), Valdeolmos, 28130 Madrid, Spain
Eszter Göltl
HUN-REN Veterinary Medical Research Institute (VMRI), Hungária krt. 21, 1143 Budapest, Hungary
Tibor Magyar
HUN-REN Veterinary Medical Research Institute (VMRI), Hungária krt. 21, 1143 Budapest, Hungary
Francesco Feliziani
National Reference Centre for Pestiviruses and Asfivirus, Istituto Zooprofilattico Sperimentale Umbria-Marche “Togo Rosati”, Via Gaetano Salvemini, 1, 06126 Perugia, Italy
Zoltán Zádori
HUN-REN Veterinary Medical Research Institute (VMRI), Hungária krt. 21, 1143 Budapest, Hungary
Lv17/WB/Rie1-Δ24 was produced via illegitimate recombination mediated by low-dilution serial passage in the Cos7 cell line and isolated on PAM cell culture. The virus contains a huge ~26.4 Kb deletion in the left end of its genome. Lv17/WB/Rie1-ΔCD-ΔGL was generated via homologous recombination, crossing two ASFV strains (Lv17/WB/Rie1-ΔCD and Lv17/WB/Rie1-ΔGL containing eGFP and mCherry markers) during PAM co-infection. The presence of unique parental markers in the Lv17/WB/Rie1-ΔCD-ΔGL genome indicates at least two recombination events during the crossing, suggesting that homologous recombination is a relatively frequent event in the ASFV genome during replication in PAM. Pigs infected with Lv17/WB/Rie1-Δ24 and Lv17/WB/Rie1/ΔCD-ΔGL strains have shown mild clinical signs despite that ASFV could not be detected in their sera until a challenge infection with the Armenia/07 ASFV strain. The two viruses were not able to induce protective immunity in pigs against a virulent Armenia/07 challenge.
