De Novo Transcriptomic Analyses Revealed Some Detoxification Genes and Related Pathways Responsive to Noposion Yihaogong<sup>®</sup> 5% EC (Lambda-Cyhalothrin 5%) Exposure in <i>Spodoptera frugiperda</i> Third-Instar Larvae
Muhammad Hafeez,
Xiaowei Li,
Zhijun Zhang,
Jun Huang,
Likun Wang,
Jinming Zhang,
Sakhawat Shah,
Muhammad Musa Khan,
Fei Xu,
G. Mandela Fernández-Grandon,
Myron P. Zalucki,
Yaobin Lu
Affiliations
Muhammad Hafeez
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Xiaowei Li
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Zhijun Zhang
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Jun Huang
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Likun Wang
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Jinming Zhang
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Sakhawat Shah
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Muhammad Musa Khan
Key Laboratory of Bio-Pesticide Innovation and Application, South China Agricultural University, Guangzhou 510642, China
Fei Xu
Central Laboratory of Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
G. Mandela Fernández-Grandon
Natural Resources Institute, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UK
Myron P. Zalucki
School of Biological Sciences, The University of Queensland, Brisbane, QLD 4072, Australia
Yaobin Lu
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a polyphagous, invasive insect pest which causes significant losses in important crops wherever it has spread. The use of pesticides in agriculture is a key tool in the management of many important crop pests, including S. frugiperda, but continued use of insecticides has selected for various types of resistance, including enzyme systems that provide enhanced mechanisms of detoxification. In the present study, we analyzed the de novo transcriptome of S. frugiperda larvae exposed to Noposion Yihaogong® 5% emulsifiable concentrate (EC) insecticide focusing on detoxification genes and related pathways. Results showed that a total of 1819 differentially expressed genes (DEGs) were identified in larvae after being treated with Noposion Yihaogong® 5% EC insecticide, of which 863 were up- and 956 down-regulated. Majority of these differentially expressed genes were identified in numerous Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including metabolism of xenobiotics and drug metabolism. Furthermore, many of S. frugiperda genes involved in detoxification pathways influenced by lambda-cyhalothrin stress support their predicted role by further co-expression network analysis. Our RT-qPCR results were consistent with the DEG’s data of transcriptome analysis. The comprehensive transcriptome sequence resource attained through this study enriches the genomic platform of S. frugiperda, and the identified DEGs may enable greater molecular underpinnings behind the insecticide-resistance mechanism caused by lambda-cyhalothrin.
