Synthesis of Mesoporous Silica Coated Gold Nanorods Loaded with Methylene Blue and Its Potentials in Antibacterial Applications
Adrián Fernández-Lodeiro,
Jamila Djafari,
Javier Fernández-Lodeiro,
Maria Paula Duarte,
Elisabete Muchagato Mauricio,
José Luis Capelo-Martínez,
Carlos Lodeiro
Affiliations
Adrián Fernández-Lodeiro
BIOSCOPE Research Group, LAQV-REQUIMTE, Chemistry Department, NOVA School of Science and Technology, FCT NOVA, NOVA University Lisbon, 2829-516 Lisbon, Portugal
Jamila Djafari
BIOSCOPE Research Group, LAQV-REQUIMTE, Chemistry Department, NOVA School of Science and Technology, FCT NOVA, NOVA University Lisbon, 2829-516 Lisbon, Portugal
Javier Fernández-Lodeiro
BIOSCOPE Research Group, LAQV-REQUIMTE, Chemistry Department, NOVA School of Science and Technology, FCT NOVA, NOVA University Lisbon, 2829-516 Lisbon, Portugal
Maria Paula Duarte
MEtRICs/DCTB/NOVA, School of Science and Technology, NOVA University Lisbon, Caparica Campus, 2829-516 Almada, Portugal
Elisabete Muchagato Mauricio
CBIOS/DREAMS, Universidade Lusófona de Humanidades e Tecnologia, Campo Grande, 376, 1749-024 Lisboa, Portugal
José Luis Capelo-Martínez
BIOSCOPE Research Group, LAQV-REQUIMTE, Chemistry Department, NOVA School of Science and Technology, FCT NOVA, NOVA University Lisbon, 2829-516 Lisbon, Portugal
Carlos Lodeiro
BIOSCOPE Research Group, LAQV-REQUIMTE, Chemistry Department, NOVA School of Science and Technology, FCT NOVA, NOVA University Lisbon, 2829-516 Lisbon, Portugal
In this work, the successful preparation and characterization of gold nanorods (AuNRs) coated with a mesoporous silica shell (AuNRs@Simes) was achieved. Conjugation with methylene blue (MB) as a model drug using ultrasound-stimulated loading has been explored for further application in light-mediated antibacterial studies. Lyophilization of this conjugated nanosystem was analyzed using trehalose (TRH) as a cryogenic protector. The obtained stable dry formulation shows potent antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria after a simple post-treatment irradiation method with a red laser during a short time period.
