Gene regulators associating the T2DM and periodontitis contributing disease prognostic markers and therapeutic target

Chanchal Katariya; G. Kaarthikeyan; Paramasivam; Mrim M. Alnfiai; Ahmed Alamoudi; Maha A Bahammam; Ammar Almarghlani; Talal M Zahid; Khalid J. Alzahrani; Hamsa Jameel Banjer; Fuad M. Alzahrani; Mohammad Assaggaf; Hosam Ali Baeshen; Mazen F. Alkahtany; Shankargouda Patil

Journal of King Saud University: Science (Feb 2023)

Gene regulators associating the T2DM and periodontitis contributing disease prognostic markers and therapeutic target

Chanchal Katariya,
G. Kaarthikeyan,
Paramasivam,
Mrim M. Alnfiai,
Ahmed Alamoudi,
Maha A Bahammam,
Ammar Almarghlani,
Talal M Zahid,
Khalid J. Alzahrani,
Hamsa Jameel Banjer,
Fuad M. Alzahrani,
Mohammad Assaggaf,
Hosam Ali Baeshen,
Mazen F. Alkahtany,
Shankargouda Patil

Affiliations

Chanchal Katariya: Department of Periodontics, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences, (SIMATS), Saveetha University, Chennai 602105, India
G. Kaarthikeyan: Department of Periodontics, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences, (SIMATS), Saveetha University, Chennai 602105, India
Paramasivam: Department of Pharmacology, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences, (SIMATS), Saveetha University, Chennai 602105, India
Mrim M. Alnfiai: Department of Information Technology, College of Computers and Information Technology, Taif University, Taif P.O. Box 11099, Taif 21944, Saudi Arabia
Ahmed Alamoudi: Oral Biology Department, King Abdulaziz University, Jeddah 21589, Saudi Arabia
Maha A Bahammam: Department of Periodontology, Faculty of Dentistry, King Abdulaziz University. Jeddah 21589, Saudi Arabia; Executive Presidency of Academic Affairs, Saudi Commission for Health Specialties, Riyadh 11614, Saudi Arabia
Ammar Almarghlani: Department of Periodontics, Faculty of Dentistry, King Abdulaziz University, Jeddah 21589, Saudi Arabia
Talal M Zahid: Periodontology Department, Faculty of Dentistry, King Abdulaziz University, Jeddah 21589, Saudi Arabia
Khalid J. Alzahrani: Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Hamsa Jameel Banjer: Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Fuad M. Alzahrani: Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Mohammad Assaggaf: Department of Periodontics, Faculty of Dentistry, King Abdulaziz University, Jeddah 21589, Saudi Arabia
Hosam Ali Baeshen: Department of Orthodontics, College of Dentistry, King Abdulziz University, Jeddah 21589, Saudi Arabia
Mazen F. Alkahtany: Department of RDS, Division of Endodontics, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia
Shankargouda Patil: College of Dental Medicine, Roseman University of Health Sciences, South Jordan, UTAH 84095, USA; Centre of Molecular Medicine and Diagnostics (COMManD), Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 600 077, India; Corresponding author.

Journal volume & issue: Vol. 35, no. 2
p. 102469

Abstract

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Periodontitis can be exacerbated by variety of systemic disorders including type 2 diabetes. T2DM patients exhibited a threefold increased risk of periodontitis. The mechanism through which T2DM induces periodontitis is uncertain. This study aims to identify co-regulatory molecules involved in T2DM periodontitis. Understanding the mechanisms could lead to new prognostic biomarkers and therapeutic targets. Methodology: Gene expression datasets (GSE156993)with experimental conditions: 1) healthy control 2) periodontitis, and 3). T2DM with periodontitis were assessed. The up-regulated genes in Group1 (healthy control vs periodontitis) and Group2 (healthy control vs T2DM with periodontitis)were identified. Protein interaction network were constructed from up-regulated genes and the topological characteristics network were determined. Each network's hubs containing the transcription factor that was up-regulated, as well as its coregulators were retrieved. Finally, the hubs were enriched based on the gene ontology and molecular pathways to recognize thehubs function. Results: 751 genes in Group1 and 762 in Group2 were noticed up-regulated. Two individual protein interaction network constructed from theseup-regulated genesthat showed complex interactions based on topological analysis. Theextracted hubs from each network displayed a variety of pathways relevant to the immune system, cell cycle, and signal processing. Analysis of hubs showed FOS transcription factor was common in both conditions containing diverse transcriptional coregulators such PKP2, RUNX2, TSC22D3, SUPT6H, and MAP3K7 in periodontitis and IDS, OPTN, PPP1R12A, BATF, and LMNA in T2DM with periodontitis. Conclusion: Our findings demonstrate FOS has a variety of regulatory patterns, providing evidence of T2M progression to periodontitis. Further experiments are still needed to confirm these transcriptional coregulators as prognostic biomarkers and therapeutic targets.

Published in Journal of King Saud University: Science

ISSN: 1018-3647 (Print)
Publisher: Elsevier
Country of publisher: Saudi Arabia
LCC subjects: Science: Science (General)
Website: http://www.journals.elsevier.com/journal-of-king-saud-university-science/

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