Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system

Robin Canac; Amandine Caillaud; Bastien Cimarosti; Aurore Girardeau; Hanan Hamamy; Bruno Reversade; Carine Bonnard; Zeina R. Al Sayed; Laurent David; Jeremie Poschmann; Patricia Lemarchand; Guillaume Lamirault; Nathalie Gaborit

Stem Cell Research (Jan 2022)

Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system

Robin Canac,
Amandine Caillaud,
Bastien Cimarosti,
Aurore Girardeau,
Hanan Hamamy,
Bruno Reversade,
Carine Bonnard,
Zeina R. Al Sayed,
Laurent David,
Jeremie Poschmann,
Patricia Lemarchand,
Guillaume Lamirault,
Nathalie Gaborit

Affiliations

Robin Canac: Université de Nantes, CHU Nantes, CNRS, INSERM, l’institut du thorax, F-44000 Nantes, France
Amandine Caillaud: Université de Nantes, CHU Nantes, CNRS, INSERM, l’institut du thorax, F-44000 Nantes, France
Bastien Cimarosti: Université de Nantes, CHU Nantes, CNRS, INSERM, l’institut du thorax, F-44000 Nantes, France
Aurore Girardeau: Université de Nantes, CHU Nantes, CNRS, INSERM, l’institut du thorax, F-44000 Nantes, France
Hanan Hamamy: Department of Genetic Medicine and Development, Geneva University, Geneva 1211, Switzerland
Bruno Reversade: Genome Institute of Singapore, A*STAR, Singapore, Singapore; Medical Genetics Department, Koç University School of Medicine (KUSOM), Istanbul, Turkey; Department of Paediatrics, National, University of Singapore, Singapore, Singapore; Institute of Molecular and Cell Biology, A*STAR, Singapore, Singapore; Reproductive Biology Laboratory, Amsterdam UMC, Amsterdam-Zuidoost, the Netherlands
Carine Bonnard: Skin Research Institute of Singapore, A*STAR, Singapore, Singapore
Zeina R. Al Sayed: Université de Nantes, CHU Nantes, CNRS, INSERM, l’institut du thorax, F-44000 Nantes, France
Laurent David: Université de Nantes, CHU Nantes, INSERM, CNRS, SFR Santé, FED 4203, INSERM UMS 016, CNRS, UMS 3556, F-44000 Nantes, France; Université de Nantes, CHU Nantes, INSERM, CRTI, UMR 1064, ITUN, F-44000 Nantes, France
Jeremie Poschmann: Université de Nantes, CHU Nantes, INSERM, CRTI, UMR 1064, ITUN, F-44000 Nantes, France
Patricia Lemarchand: Université de Nantes, CHU Nantes, CNRS, INSERM, l’institut du thorax, F-44000 Nantes, France
Guillaume Lamirault: Université de Nantes, CHU Nantes, CNRS, INSERM, l’institut du thorax, F-44000 Nantes, France
Nathalie Gaborit: Université de Nantes, CHU Nantes, CNRS, INSERM, l’institut du thorax, F-44000 Nantes, France; Corresponding author at: l'institut du thorax Inserm UMR 1087, CNRS, UMR 6291, IRS-UN, 8 quai Moncousu, 44007 Nantes cedex 1, France.

Journal volume & issue: Vol. 58
p. 102627

Abstract

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Studies on animal models have shown that Irx5 is an important regulator of cardiac development and that it regulates ventricular electrical repolarization gradient in the adult heart. Mutations in IRX5 have also been linked in humans to cardiac conduction defects. In order to fully characterize the role of IRX5 during cardiac development and in cardiomyocyte function, we generated three genetically-modified human induced pluripotent stem cell lines: two knockout lines (heterozygous and homozygous) and a knockin HA-tagged line (homozygous).

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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