Early LPS-induced ERK activation in retinal pigment epithelium cells is dependent on PIP2-PLC
Melina V. Mateos,
Constanza B. Kamerbeek,
Norma M. Giusto,
Gabriela A. Salvador
Affiliations
Melina V. Mateos
Corresponding author. Tel.: +54 291 4861201; fax: +54 291 4861200.; Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), Universidad Nacional del Sur (UNS) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Camino La Carrindanga km 7, 8000 Bahía Blanca, Argentina
Constanza B. Kamerbeek
Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), Universidad Nacional del Sur (UNS) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Camino La Carrindanga km 7, 8000 Bahía Blanca, Argentina
Norma M. Giusto
Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), Universidad Nacional del Sur (UNS) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Camino La Carrindanga km 7, 8000 Bahía Blanca, Argentina
Gabriela A. Salvador
Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), Universidad Nacional del Sur (UNS) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Camino La Carrindanga km 7, 8000 Bahía Blanca, Argentina
This article presents additional data regarding the study “The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium” [1]. The new data presented here show that short exposure of RPE cells to lipopolysaccharide (LPS) induces an early and transient activation of the extracellular signal-regulated kinase (ERK1/2). This early ERK1/2 activation is dependent on phosphatidylinositol bisphosphate-phospholipase C (PIP2-PLC). On the contrary, neither the phospholipase D 1 (PLD1) nor the PLD2 inhibition is able to modulate the early ERK1/2 activation induced by LPS in RPE cells. Keywords: Retinal pigment epithelium (RPE), Inflammation, Phosphatidylinositol bisphosphate-phospholipase C (PIP2-PLC), Phospholipase D (PLD), Extracellular signal-regulated kinase (ERK1/2), Lipopolysaccharide (LPS)
