Ciência Florestal (Jan 2014)

EFEITO DO INTERVALO DE IMERSÃO E DE INJEÇÃO DE AR NA MULTIPLICAÇÃO IN VITRO DE Eucalyptus grandis x Eucalyptus urophylla EM BIORREATOR DE IMERSÃO TEMPORÁRIA

  • Mila Liparize de Oliveira,
  • Aloisio Xavier,
  • Ricardo Miguel Penchel Filho,
  • Jocemar Palauro dos Reis

Journal volume & issue
Vol. 24, no. 1
pp. 37 – 45

Abstract

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The objectives of this study were to evaluate different immersion frequencies (2, 4, 8 and 16 h) and explants supports (filter paper and foam) of a Eucalyptus grandis x Eucalyptus urophylla clone inside RITA ® bioreactors; and a ventilation system with additional air input coupled to the bioreactor containers. It was evaluated the explants fresh weight, number of shoots and hyperhydricity and in vitro axillary bud multiplication. The immersions every two and four hours and the filter paper showed higher growth and number of shoots, but caused a greater percentage of hyperhydric shoots. Under the conditions and system tested, the additional air injection in the RITA ® bioreactor did not influence the culture growth.