TOLL-LIKE RECEPTOR-4 (TLR4) GENE POLYMORPHISM IN HARINGHATA BLACK CHICKEN BREED

Abstract

The experiment was conducted on Haringhata Black Chicken breed to investigate the polymorphism of exon-2 of Toll-Like Receptor-4 gene. Genomic DNA extracted from 82 birds was used for Polymerase Chain Reaction (PCR). Amplified polymerase chain reaction (PCR) product (248bp) of exon-2 of TLR4 gene revealed two types of SSCP banding pattern arbitrarily assigned as EF and FF genotypes on 10 percent polyacrylamide gel. The genotype frequencies were found to be 0.634 for EF and 0.366 for FF genotype with respect to exon-2 of TLR4 gene. The frequencies of E and F alleles were estimated as 0.317 and 0.683, respectively. The calculated Chi square value revealed that the population was not in Hardy Weinberg Equilibrium with respect to TLR4 gene in Haringhata Black Chicken.

Keywords

• Haringhata Black Chicken
• TLR4 Gene
• Polymorphism
• PCR-SSCP