A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples

Xiaojun Wei; Xiaoqin Wang; Zehui Zhang; Yuanyuan Luo; Zixin Wang; Wen Xiong; Piyush K. Jain; John R. Monnier; Hui Wang; Tony Y. Hu; Chuanbing Tang; Helmut Albrecht; Chang Liu

doi:10.1038/s41467-022-34273-x

Nature Communications (Nov 2022)

A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples

Xiaojun Wei,
Xiaoqin Wang,
Zehui Zhang,
Yuanyuan Luo,
Zixin Wang,
Wen Xiong,
Piyush K. Jain,
John R. Monnier,
Hui Wang,
Tony Y. Hu,
Chuanbing Tang,
Helmut Albrecht,
Chang Liu

Affiliations

Xiaojun Wei: Biomedical Engineering Program, University of South Carolina
Xiaoqin Wang: Department of Chemical Engineering, University of South Carolina
Zehui Zhang: Biomedical Engineering Program, University of South Carolina
Yuanyuan Luo: Department of Chemistry and Biochemistry, University of South Carolina
Zixin Wang: Department of Chemistry and Biochemistry, University of South Carolina
Wen Xiong: Department of Chemical Engineering, University of South Carolina
Piyush K. Jain: Department of Chemical Engineering, University of Florida
John R. Monnier: Department of Chemical Engineering, University of South Carolina
Hui Wang: Department of Chemistry and Biochemistry, University of South Carolina
Tony Y. Hu: Center for Cellular and Molecular Diagnostics, Tulane University School of Medicine
Chuanbing Tang: Department of Chemistry and Biochemistry, University of South Carolina
Helmut Albrecht: Department of Internal Medicine, School of Medicine, University of South Carolina
Chang Liu: Biomedical Engineering Program, University of South Carolina

DOI: https://doi.org/10.1038/s41467-022-34273-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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Accurate detection of antigen p24 for HIV−1 early diagnosis remains challenging. Here the authors present a click chemistry amplified nanopore (CAN) assay that allows p24 quantification at ultralow concentration range in clinical samples.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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