Plant Methods (Aug 2017)

Quantification of seed–soil contact of sugar beet (Beta vulgaris) using X-ray Computed Tomography

  • Sebastian Blunk,
  • Ali Hafeez Malik,
  • Martine I. de Heer,
  • Tobias Ekblad,
  • Jennifer Bussell,
  • Debbie Sparkes,
  • Kenneth Fredlund,
  • Craig J. Sturrock,
  • Sacha J. Mooney

DOI
https://doi.org/10.1186/s13007-017-0220-4
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 14

Abstract

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Abstract Background Seed–soil contact is important to ensure successful germination, however, there is a paucity of reported studies that have quantified the microstructure at and around this critical interface, mainly due to the opacity of soil. Results Here we describe a novel methodology to non-destructively calculate the seed–soil contact area using X-ray Computed Tomography. Under controlled conditions, we observed that seed–soil contact was strongly influenced by the size and type of seed, with a seed–soil contact of ca. 15% for naked sugar beet seeds compared to ca. 32% for pelleted and coated seeds. Similar results were obtained for seeds sampled from the field albeit with a higher spatial variability. Conclusions By application of this new quantification method it is hoped seed enhancement technologies can be optimised and ultimately seedbed preparation improved to ensure better germination.

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