Evaluation of antioxidant activity for pure curcumin in albino mice

Abstract

he study was carried out in order to determine the toxic, oxidant and antioxidant effects for one compounds of the Curcrcuma longa L. in comparison with vitamin C against the mutagenic effect of carbon tetrachloride CCl4, which is a chemical compound that damages hepatic cells and has mutagenic effects. The effect was studied in a mammalian system. This in vivo system depended on evaluating the enzymatic activity of two anti oxidant enzymes: Catalase and Glutathione reductase. Two concentrations of pure curcumin 200 and 400 mg /kg were evaluated to chose the suitable concentration, which remembered the negative control. In order be used in The interaction experiments, included two types of treatments pre – CCl4 and post-CCl4 in order to determine the mechanisms of the pure curcumin in preventing or reducing the antioxidant effect of CCl4. The pure curcumin showed no toxic and oxidant effects in biological system, and instead it showed highly performance in preventing or reducing the oxidant stress effects of CCl4. It increased the Catalase and Glutathione reductase antioxidant activity, especially in dose 200 mg/kg. The positive effect was higher when pure curcumin was used as pre-CCl4 treatments, and to less extent in post-CCl4 treatment. Therefore, the plant extracts can be considered as adesmutagen at the first degree and a bioantimutagen at the second degree.