Development and application of a 1K functional liquid chip for lactation performance in Bactrian camels

Lili Guo; Lili Guo; Lili Guo; Lema Dao; Bin Liu; Jingyu Wang; Zaixia Liu; Zaixia Liu; Fengying Ma; Fengying Ma; Bielige Morigen; Chencheng Chang; Chencheng Chang; Yinbatu Bai; Yinbatu Bai; Yaqiang Guo; Yaqiang Guo; Caixia Shi; Caixia Shi; Junwei Cao; Junwei Cao; Wenguang Zhang; Wenguang Zhang; Wenguang Zhang

doi:10.3389/fvets.2024.1359923

Frontiers in Veterinary Science (Jul 2024)

Development and application of a 1K functional liquid chip for lactation performance in Bactrian camels

Lili Guo,
Lili Guo,
Lili Guo,
Lema Dao,
Bin Liu,
Jingyu Wang,
Zaixia Liu,
Zaixia Liu,
Fengying Ma,
Fengying Ma,
Bielige Morigen,
Chencheng Chang,
Chencheng Chang,
Yinbatu Bai,
Yinbatu Bai,
Yaqiang Guo,
Yaqiang Guo,
Caixia Shi,
Caixia Shi,
Junwei Cao,
Junwei Cao,
Wenguang Zhang,
Wenguang Zhang,
Wenguang Zhang

Affiliations

Lili Guo: College of Life Science, Inner Mongolia Agricultural University, Hohhot, China
Lili Guo: Inner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Inner Mongolia Agricultural University, Hohhot, China
Lili Guo: Inner Mongolia Autonomous Region Key Laboratory of Biomanufacturing, Hohhot, China
Lema Dao: Bactrian Camel Institute of Alsha, Bayanhot, China
Bin Liu: Inner Mongolia Bionew Technology Co., Ltd., Hohhot, China
Jingyu Wang: Bactrian Camel Institute of Alsha, Bayanhot, China
Zaixia Liu: Inner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Inner Mongolia Agricultural University, Hohhot, China
Zaixia Liu: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Fengying Ma: Inner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Inner Mongolia Agricultural University, Hohhot, China
Fengying Ma: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Bielige Morigen: Animal Disease Prevention and Control Center of Alsha, Bayanhot, China
Chencheng Chang: Inner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Inner Mongolia Agricultural University, Hohhot, China
Chencheng Chang: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Yinbatu Bai: Inner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Inner Mongolia Agricultural University, Hohhot, China
Yinbatu Bai: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Yaqiang Guo: Inner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Inner Mongolia Agricultural University, Hohhot, China
Yaqiang Guo: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Caixia Shi: Inner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Inner Mongolia Agricultural University, Hohhot, China
Caixia Shi: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Junwei Cao: College of Life Science, Inner Mongolia Agricultural University, Hohhot, China
Junwei Cao: Inner Mongolia Autonomous Region Key Laboratory of Biomanufacturing, Hohhot, China
Wenguang Zhang: College of Life Science, Inner Mongolia Agricultural University, Hohhot, China
Wenguang Zhang: Inner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Inner Mongolia Agricultural University, Hohhot, China
Wenguang Zhang: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China

DOI: https://doi.org/10.3389/fvets.2024.1359923
Journal volume & issue: Vol. 11

Abstract

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IntroductionThe advancement of high-throughput, high-quality, flexible, and cost-effective genotyping platforms is crucial for the progress of dairy breeding in Bactrian camels. This study focuses on developing and evaluating a 1K functional liquid single nucleotide polymorphism (SNP) array specifically designed for milk performance in Bactrian camels.MethodsWe utilized RNA sequencing data from 125 lactating camels to identify and select 1,002 loci associated with milk production traits for inclusion in the SNP array. The array’s performance was then assessed using 24 randomly selected camels. Additionally, the array was employed to genotype 398 individuals, which allowed for population validation to assess the polymorphism of SNP sites.ResultsThe SNP array demonstrated high overall SNP call rates (> 99%) and a remarkable 100% consistency in genotyping. Population validation results indicate that camels from six breeding areas in Northwest China share a similar genetic background regarding lactation functionality.DiscussionThis study highlights the potential of the SNP array to accelerate the breeding process of lactating Bactrian camels and provides a robust technical foundation for improving lactation performance.

Published in Frontiers in Veterinary Science

ISSN: 2297-1769 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: https://www.frontiersin.org/journals/veterinary-science

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