THE ROLE OF MAST CELLS DEGRANULATION REACTION IN THE ADJUSTMENT OF INSULIN IN DIABETES MELLITUS PATIENTS

Abstract

Introduction: Strategy of treatment of patients with diabetes (DM) in Belarus is based on a basis of domestic and international conciliatory documents in the area of diabetes. Patients with DM apply domestic production insulin, and at development of individual intolerance or allergic reaction, there is a possibility to replace applied insulin other less antigenic preparation. Aim: To conduct a reaction clinics-experimental research based on mast cells degranulation corpulent cages (RDMC), arising on various kinds of the insulin applied in republic of Belarus. Material and methods: Estimation RDMC of animals on various kinds of insulin which received 31 patient with DM with presence in the anamnesis of various allergic reactions and necessity for correction insulin therapy or change of a preparation of insulin. Monitoring fasting and postprandial glycemia was carried out, degree of indemnification of a carbohydrate exchange was estimated. The applied technique consists in revealing RDMC of a rat at incubation with whey of the patient and investigated insulin. At presence in whey of the patient specific IgЕ to the given kind of insulin occurs degranulation corpulent cages. Results: More than 80 % of patients had sub- or decompensation of a diabetes. Sharply positive tests (RDMC more than 40 %) are absent. On 5 entered preparations of insulin positive reactions (3-13 %) are fixed. All preparations of insulin had weakly positive RDMC (29-55 %). Сonclusion: Carrying out RDMC on various kinds of insulin will help the clinical physician to pick up less immune medical product in case of presence at the patient of allergic reaction. All preparations of insulin investigated in given work can be applied to complex treatment DM.

Keywords

• diabetes mellitus
• insulin
• mast cell degranulation reaction