Superoxide dismutase and catalase activities in the liver and muscle of barbel (Barbus barbus) and its intestinal parasite (Pomphoryinchus laevis) from the Danube river, Serbia

Abstract

The activities of total superoxide dismutase (Tot-SOD), manganese-containing superoxide dismutase (Mn-SOD), copper zinc containing superoxide dismutase (Cu/Zn-SOD) and catalase (CAT) protein concentration, as well as protein and SOD electrophoretic profiles in the liver and muscle of barbel (Barbus barbus) and its intestinal parasite Pomphoryinchus laevis from the river Danube, within the suburban area of Belgrade, Serbia (the stretch between Višnjica and Grocka) in spring and summer were investigated. Specific activities of Tot-SOD, Mn-SOD, Cu/Zn-SOD were higher in spring, while specific CAT activity was higher in summer in all investigated samples. Temperature influence on the antioxidant defense enzymes in barbel tissue and in its intestinal parasite Pomphoryinchus laevis, as well as seasonal patterns, are evident. Our work represents the first study of SOD and CAT activities in the barbel and its intestinal parasites and shows that barbel and acanthocephalans are very useful for biomonitoring studies in aquatic ecosystems.

Keywords

• Barbel
• parasite
• liver
• muscle
• biomarkers
• oxidative stress
• antioxidant enzymes
• season