The effect of nanoscale biodegradable polymer film on the healing of cut wounds of the skin of complicated Psevdomonas auregenosa (Experimental research)

Abstract

Introduction. Effect of the biodegradable polymer film saturated with zinc nanoxide and hydrated C60 fullerene on the healing of skin cut wounds complicated Pseudomonas aeruginosa are study. Materials and methods. The studies were performed on 35 Guinea pigs in vivarium of IvanoFrankivsk National Medical University. The animals were divided into three groups: group I (10 animals) the wounds were treated without the use of films; group II (10 animals) – the covering material for Curasorb Zn wounds was used; group III (10 animals) –the biodegradable polymer film with C60 fullerene and zinc nanoxide (nZnO – particle size of 30 nm) was used. The simulated skin cut wounds were infected with Pseudomonas aeruginosa and investigated wound healed at 3, 7, 14 and 21 days using a Curasorb Zn film and a biodegradable polymer film with C60 fullerene and zinc nanoxide. The area of the wounds was measured, the microbiological examination of the wound content and pathomorphological evaluation of the wound tissues. Results. Wounds area in animals of group II to the 3-rd day increased slightly by 8,9 mm2, and wounds area animals of group III decreased by 44,8 mm2, indicating the therapeutic effect and sorption capacity of the film from the first days of treatment. Curasorb Zn films reduced the bacterial pathogen by two orders and the nanooxide film reduced the bacterial level to non-pathogenic levels of magnitude by 3 orders of magnitude by day 3. The bacterial level remained high in the film-free group (group I). Conclusion. The polymer film saturated with zinc nanooxide and hydrated C60 fullerene has a high local wound healing effect, antimicrobial activity, which improves the effectiveness of local treatment of purulent wounds and reduce the healing time.

Keywords

• purulent wound
• polymer films
• local treatment