Study of angiotropic activity of the nerve growth factor in experiments on human endothelial cell culture (HUVEC)

Abstract

Resume. In vitro experiments performed on human endothelial ceLL culture (HUVEC), nerve growth factor (NGF) angiotropic effects were studied. An intact ceLL culture it was shown that NGF exhibited angiogenic properties and stimulated the initial stage of angiogenesis -tubuLogenesis at a 10-9 M concentration, as evidenced by a statisticaLLy significant increase in the average totaL Length of microtubuLes compared with the control. Under conditions of oxidative stress, NGF neutraLizes the damaging effect of hydrogen peroxide in a high concentration (200 |±M) and not onLy shows an angioprotective effect, but aLso prevents the deveLopment of morphoLogicaL changes in nucLear chromatin, characteristic for apoptosis. Under these conditions NGF (10-9 M) reduces statisticaLLy significant by comparison with the controL the number of ceLLs with condensed and fragmented chromatin: 44 ± 9 and 78 ± 9 (р ≤ 0,05), respectiveLy. The obtained resuLts confirm the data about the NGF angiogenic activity and indicate that under conditions of the vesseLs endotheLiaL ceLLs damage by oxidative stress, it exhibits pronounced angioprotective and anti-apoptotic activity.

Keywords

• фактор роста нерва
• клетки huvec
• перекись водорода
• оксидативный стресс
• ангиопротекторная активность
• антиапоптотическая
• nerve growth factor
• huvec cells
• hydrogen peroxide
• oxidative stress
• angioprotective activity
• antiapoptotic activity