Prime Vaccination with Chitosan-Coated Phipps BCG and Boosting with CFP-PLGA against Tuberculosis in a Goat Model
Yesenia Guadalupe Contreras-Magallanes,
Marina Durán-Aguilar,
Susana L. Sosa-Gallegos,
Ángel H. Álvarez,
Fátima A. Andrade-Santillán,
Isabel Bárcenas-Reyes,
Sara González-Ruíz,
Elba Rodríguez-Hernández,
Germinal J. Cantó-Alarcón,
Feliciano Milián-Suazo
Affiliations
Yesenia Guadalupe Contreras-Magallanes
Doctorado en Ciencias Biológicas, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76230 Querétaro, Mexico
Marina Durán-Aguilar
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76239 Querétaro, Mexico
Susana L. Sosa-Gallegos
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76239 Querétaro, Mexico
Ángel H. Álvarez
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Av. Normalistas 800, Col. Colinas de la Normal, 44370 Guadalajara, Mexico
Fátima A. Andrade-Santillán
Maestría en Salud y Producción Animal Sustentable, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N Juriquilla, Delegación Santa Rosa Jáuregui, 78230 Querétaro, Mexico
Isabel Bárcenas-Reyes
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76239 Querétaro, Mexico
Sara González-Ruíz
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76239 Querétaro, Mexico
Elba Rodríguez-Hernández
Centro Nacional de Investigación Disciplinaria en Fisiología y Mejoramiento Animal, INIFAP, SAGARPA, Km. 1 Carretera a Colón, Ajuchitlan, 76280 Colón, Mexico
Germinal J. Cantó-Alarcón
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76239 Querétaro, Mexico
Feliciano Milián-Suazo
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76239 Querétaro, Mexico
Attempts to improve the immune response and efficacy of vaccines against tuberculosis in cattle, goats, and other animal species have been the focus of research in this field during the last two decades. Improving the vaccine efficacy is essential prior to running long-lasting and expensive field trials. Studies have shown that vaccine protocols utilizing boosting with proteins improve the vaccine efficacy. The use of polymers such as chitosan and PolyLactic-co-Glycolic Acid (PLGA) improves the immune response against different diseases by improving the interaction of antigens with the cellular immune system and modulating the host immune response. This study shows that the prime BCG vaccination, boosted with a culture filtrate protein (CFP), alone or in combination with chitosan and PolyLactic-co-Glycolic Acid (PLGA), have the potential to reduce tuberculosis (TB) dissemination by reducing the number of animals with lesions, the number of lesions per animal, and the size of the lesions in vaccinated animals, compared with those not vaccinated or those vaccinated with BCG alone. The vaccinated groups showed significantly higher Interferon-γ levels in the blood compared to the control, nonvaccinated group after vaccination, after boosting, and after the challenge with the wild-type Mycobacterium bovis strain.
