In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument

Yuezhu Wang; Yichi Zhang; Junsheng Wang; Weibing Liu; Huan Wang; Mingzhu Song; Hongyue Zhang; Xin Wang

Ecotoxicology and Environmental Safety (Jan 2024)

In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument

Yuezhu Wang,
Yichi Zhang,
Junsheng Wang,
Weibing Liu,
Huan Wang,
Mingzhu Song,
Hongyue Zhang,
Xin Wang

Affiliations

Yuezhu Wang: Liaoning Key Laboratory of Marine Sensing and Intelligent Detection, Dalian Maritime University, 116026 Dalian, China; College of Environmental Sciences and Engineering, Dalian Maritime University, 116026 Dalian, China
Yichi Zhang: Liaoning Key Laboratory of Marine Sensing and Intelligent Detection, Dalian Maritime University, 116026 Dalian, China; Information Science and Technology College, Dalian Maritime University, 116026 Dalian, China
Junsheng Wang: Liaoning Key Laboratory of Marine Sensing and Intelligent Detection, Dalian Maritime University, 116026 Dalian, China; Information Science and Technology College, Dalian Maritime University, 116026 Dalian, China; Corresponding author at: Liaoning Key Laboratory of Marine Sensing and Intelligent Detection, Dalian Maritime University, 116026 Dalian, China.
Weibing Liu: The people's Hospital of Liaoning Province, 110067 Shenyang, China
Huan Wang: Department of Thoracic Surgery, Liaoning Cancer Hospital & Institute, 110801 Shenyang, China
Mingzhu Song: Liaoning Key Laboratory of Marine Sensing and Intelligent Detection, Dalian Maritime University, 116026 Dalian, China; Information Science and Technology College, Dalian Maritime University, 116026 Dalian, China
Hongyue Zhang: Liaoning Key Laboratory of Marine Sensing and Intelligent Detection, Dalian Maritime University, 116026 Dalian, China; Information Science and Technology College, Dalian Maritime University, 116026 Dalian, China
Xin Wang: Liaoning Key Laboratory of Marine Sensing and Intelligent Detection, Dalian Maritime University, 116026 Dalian, China; Information Science and Technology College, Dalian Maritime University, 116026 Dalian, China

Journal volume & issue: Vol. 270
p. 115894

Abstract

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Algal toxins produced by microalgae, such as domoic acid (DA)11 (DA) domoic acid, have toxic effects on humans. However, toxicity tests using mice only yield lethal doses of algal toxins without providing insights into the mechanism of action on cells. In this study, a fast segmentation of microfluidic flow cytometry cell images based on the bidirectional background subtraction (BBS)22 (BBS) bidirectional background subtraction method was developed to get the visual evidence of apoptosis in both bright-field and fluorescence images. This approach enables mapping of changes in cell morphology and activity under algal toxins, allowing for fast (within 60 s) and automated biological detection. By combining microfluidics with flow cytometry, the intricate cellular-level reaction process can be observed in micro samples of 293 T cells and mouse spleen cells, offering potential for future in vitro experiments.

Published in Ecotoxicology and Environmental Safety

ISSN: 0147-6513 (Print); 1090-2414 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Technology: Environmental technology. Sanitary engineering: Environmental pollution; Geography. Anthropology. Recreation: Environmental sciences
Website: https://www.journals.elsevier.com/ecotoxicology-and-environmental-safety

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