STAR Protocols (Jun 2021)
Protocol for single-cell ATAC sequencing using combinatorial indexing in mouse lung adenocarcinoma
Abstract
Summary: Single-cell ATAC sequencing using combinatorial indexing (sciATAC-seq) enables the identification of chromatin accessibility profiles at single-cell resolution with a dual-barcoding approach during transposition and library construction. Unlike commercial droplet-based approaches, sciATAC-seq is a cost-effective, extensible strategy that permits flexibility in the experimental scale via multiplexed barcoding across samples or perturbations. In contrast, droplet-based approaches have higher cell recovery and may be advantageous when cell input is limited. The step-by-step sciATAC-seq protocol described here is amenable to diverse cell types and fixed samples.For complete details on the use and execution of this protocol, please refer to LaFave et al. (2020).