Central European Journal of Immunology (Jul 2022)

Generation of high cross-presentation ability human dendritic cells by combination of interleukin 4, interferon β and GM-CSF

  • Yihua Pi,
  • Yifang Li,
  • Rongyi Liang,
  • Jian Xiao,
  • Jing Leng,
  • Lifeng Zhang

DOI
https://doi.org/10.5114/ceji.2022.117767
Journal volume & issue
Vol. 47, no. 2
pp. 125 – 138

Abstract

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Dendritic cell (DC)-based immunotherapies have been utilized for the treatment of numerous diseases. However, the conventional generation strategies of DCs in vitro require 7 days and these DCs showed an unsatisfactory function, which prompted us to explore new approaches. We found that in vitro culture of human CD14+ cells, in the medium containing granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-4, as well as interferon  (IFN-) for 48 h, followed by the maturation stimuli of IL-1 and poly I:C for another 24 h can be differentiated into high cross-presentation ability DCs (G4B-DCs). These DCs express high levels of CD11c, CD86, and HLA-DR, producing a high level of tumor necrosis factor  (TNF-). Of note, compared with the conventional DCs, G4B-DCs showed a higher ability to promote allogeneic naïve CD4+ T cell and CD8+ T cell proliferation and interferon (IFN)- production. These DCs also have the remarkable ability to induce Flu-M1-specific CD8+ T cells. In addition, we found that these G4B-DCs express partially the cDC1 phenotype. These data indicate that G4B-DC is unique and may provide a relatively rapid alternative method for potential clinical use.

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