RELATIONSHIPS BETWEEN GINGIVAL FIBROBLASTS AND NUR77-ACTIVATED PRO-B LYMPHOCYTES

Abstract

Aim of the study We explored the relationships between the gingival fibroblasts and pro-B lymphocytes apoptosis in vitro because the current medical literature is lacking such data. Material and methods The aim of our study was represented by the effects of gingival fibroblasts on the apoptosis of Nur77 receptors-activated pro-lymphocytes in vitro, also in the presence of some retinoid derivatives. Gingival fibroblasts were obtained from male rats. Pro-B cells of murine type in cultures were used for actual experiments. The apoptosis of pro-B cells was induced using a combination of cytosporone B (50 µM) and cyclosporine A (1 µM). The apoptotic effects were revealed using the classical flow cytometry techniques. Results We clearly showed that pro-B lymphocyte apoptosis induced by the combination of cytosporone B (Nur77 agonist) and cyclosporine A in the presence of gingival fibroblasts could be slightly reduced by 13-cis retinoic acid. The inhibitory effects of 13-cis retinoic acid were powerful than those of tazarotenic acid. The effects of 13-cis retinoic acid and tazarotenic acid were evaluated toward the effects of the combination of cytosporone B and cyclosporine A. Conclusions The intrinsic pathways of retinoid derivatives remain to be established by further experiments

Keywords

• 13-cis retinoic acid
• cyclosporine a
• cytosporone b
• gingival fibroblast
• nur77
• pro-b lymphocyte