Engineering a Bi-Conical Microchip as Vascular Stenosis Model
Yan Li,
Jianchun Wang,
Wei Wan,
Chengmin Chen,
Xueying Wang,
Pei Zhao,
Yanjin Hou,
Hanmei Tian,
Jianmei Wang,
Krishnaswamy Nandakumar,
Liqiu Wang
Affiliations
Yan Li
Energy Research Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
Jianchun Wang
Energy Research Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
Wei Wan
Energy Research Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
Chengmin Chen
Energy Research Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
Xueying Wang
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China
Pei Zhao
Energy Research Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
Yanjin Hou
Energy Research Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
Hanmei Tian
Energy Research Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
Jianmei Wang
Energy Research Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
Krishnaswamy Nandakumar
Energy Research Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
Liqiu Wang
Energy Research Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
Vascular stenosis is always associated with hemodynamic changes, especially shear stress alterations. Herein, bi-conical shaped microvessels were developed through flexibly and precisely controlled templated methods for hydrogel blood-vessel-like microchip. The blood-vessel-like microvessels demonstrated tunable dimensions, perfusable ability, and good cytocompatibility. The microchips showed blood-vessel-like lumens through fine embeddedness of human umbilical vein endothelial cells (HUVECs) on the interior surface of hydrogel microchannels, which closely reproduced the morphology and functions of human blood vessels. In the gradual narrowing region of bi-conical shape, fluid flow generated wall shear stress, which caused cell morphology variations. Wall shear rates at the gradual narrowing region were simulated by FLUENT software. The results showed that our microchannels qualified for performance as a vascular stenosis-like model in evaluating blood hydrodynamics. In general, our blood-vessel-on-a-chip could offer potential applications in the prevention, diagnosis, and therapy of arterial thrombosis.
