Determination of Toxicity of Pulp-MIII Effluents by Using Allium Test

Abstract

In order to evaluate the effect of wastewater treatment process on toxicity of Kraft mili effluent, wastewater samples prior A and after the treatment B was examined by using the modified Allium test. Treatment of A. cepa bulbs with wastewater samples has caused inhibition to root growth when compared with the controls. Growth inhibition values for wastewater samples, A and B, were determined 55.72 % and 48.33 %, respectively. Treatment of root tip cells with wastewater samples led to cytological abnormalities including c-mitosis, anaphase with laggard chromosome, fragment and bridges and chromosome stickness in root-tip cells. Percentages of the c-mitosis were 47.7% and 53.3% for root-tip cells treated with A and B samples, respectively. Taking the total aberrations into account, percentages of laggard chromosomal fragment and bridge formations were 26.6% for those treated with sample A and 33.3% with sample B. Chromosome stickiness were determined 12.2% and 10.6% for samples A and B, respectively. Banded chromosome was observed at the frequency of 13.3% of aberrant cells caused by sample A. Banded chromosome and multipolar cell formation were rarely observed in treatments with sample B.

Keywords

• allium testi
• toksisite
• su kirlili ğ i
• kağı t fabrikas ı
• ar ı t ı m tesisi atı k sular ı
• mitoz
• allium test
• toxicity
• water pollution
• kraft-mili
• wastewater treatment plant
• mitosis