Xin yixue (Oct 2022)
Comparison of cytokine profile in mesenchymal stem cells derived from human umbilical cord and placenta
Abstract
Objective To compare the secretion of cytokines from human umbilical cord-derived mesenchymal stem cells （UC-MSCs） and placenta-derived mesenchymal stem cells （P-MSCs）. Methods UC-MSCs and P-MSCs were isolated and cultured. Cell surface antigens of MSCs were measured using flow cytometry. The adipogenic and osteogenic differentiation capability was analyzed by Oil red O, Von Kossa staining and Alizarin red S staining. The conditioned medium was collected and the expression of cytokines was detected by microarray. Microarray assay results were validated by ELISA and RT-PCR. Results UC-MSCs and P-MSCs had similar surface markers,adipogenic and osteogenic differentiation capacity. Both UC-MSCs and P-MSCs highly expressed insulin-like growth factor-binding protein （IGFBP）-3, IGFBP-4 and IGFBP-6, tissue inhibitor of metallo proteinase （TIMP）-1 and TIMP-2, IL-6, TGF-β1, hepatocyte growth factor, monocyte chemoattractant protein-1 and human intercellular adhesion molecule-1, whereas lowly expressed epidermal growth factor, IL-17, IL-12p40,B lymphocyte chemoattractant, tumor necrosis factor-β （TNF-β）, IL-13, MIP-1δ, Eotaxin-2, IL-12p70 and platelet-derived growth factor-BB. Compared with UC-MSCs,P-MSCscontained five differentially-expressed proteins including TNF receptorⅡ, stem cell factor, nerve growth factor receptor, TGF-β3 and IL-1β. The results of ELISA and RT-PCR were consistent with themicroarray protein data. Conclusion UC-MSCs and P-MSCs have similar surface markers, osteogenic and adipogenic differentiation capacity and cytokine profile.
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