فیزیولوژی محیطی گیاهی (Nov 2007)
Effect of drought stress and ascorbic acid on some of antioxidant enzymes in antioxidative defense in Pimpinella anisum L.
Abstract
Drought stress is one of the abiotic stresses that induces reactive oxygen species(ROS) formation in plant tissues. ROS signaling promotes complex networks of antioxidant enzymes. Ascorbic acid is an important buffer protects cellular structures against oxidative attack in plants. Effect of drought stress on catalase (CAT), ascorbate peroxidase (APX) and polyphenol oxidase (PPO) enzyme activities was evaluated in the presence and absence of ascorbate in herbal aromatic plant Pimpinella anisum L. with great value of export that exposed to controlled water deficit. This study was done in flower pot condition. Biochemical and physiological properties measured randomly in control groups and treatments (with drought 60% and 25% of field capacity and ascorbate with concentration of 1.4 mM). Results showed that with increasing stress levels in leaves CAT, APX and PPO enzyme activities increased significantly in the presence and absence of ascorbate. Activity of root CAT and APX with and without ascorbate at the level of drought 60% increased significantly and at the level 25% of field capacity decreased to the level of control. However ascorbate treatment increased significantly the root enzymatic activities in whole stress levels.
