Nature Communications (Apr 2023)

The IgG4 hinge with CD28 transmembrane domain improves VHH-based CAR T cells targeting a membrane-distal epitope of GPC1 in pancreatic cancer

  • Nan Li,
  • Alex Quan,
  • Dan Li,
  • Jiajia Pan,
  • Hua Ren,
  • Gerard Hoeltzel,
  • Natalia de Val,
  • Dana Ashworth,
  • Weiming Ni,
  • Jing Zhou,
  • Sean Mackay,
  • Stephen M. Hewitt,
  • Raul Cachau,
  • Mitchell Ho

DOI
https://doi.org/10.1038/s41467-023-37616-4
Journal volume & issue
Vol. 14, no. 1
pp. 1 – 19

Abstract

Read online

Glypican-1 (GPC1) expression is elevated in pancreatic cancer and has been exploited as a therapeutic target. Here the authors report the development of VHH nanobody-based CAR-T cells targeting GPC1, showing anti-tumor activity in pancreatic cancer preclinical models.