CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens

Alexendar R. Perez; Laura Sala; Richard K. Perez; Joana A. Vidigal

doi:10.1038/s41467-021-26722-w

Nature Communications (Nov 2021)

CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens

Alexendar R. Perez,
Laura Sala,
Richard K. Perez,
Joana A. Vidigal

Affiliations

Alexendar R. Perez: Laboratory of Biochemistry and Molecular Biology, National Cancer Institute
Laura Sala: Laboratory of Biochemistry and Molecular Biology, National Cancer Institute
Richard K. Perez: School of Medicine, University of California, San Francisco
Joana A. Vidigal: Laboratory of Biochemistry and Molecular Biology, National Cancer Institute

DOI: https://doi.org/10.1038/s41467-021-26722-w
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 11

Abstract

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Off-target effects often confound the interpretation of CRISPR screens. Here, the authors introduce a computational method that corrects for off-targets in gRNA depletion assays, circumventing the need to completely discard unspecific guides.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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