Effect of Supernatant Extract and Cell Lysate of Probiotic Yeast of Saccharomyces Cerevisiae on Biofilm and Alginate Production in Pseudomonas Aeruginosa

Abstract

Background and Aims: Pseudomonas aeruginosa is a potent pathogen for humans using multiple virulence factors. The purpose of this study was to investigate the effect of Saccharomyces cerevisiae lysates and supernatants on biofilm, alginate factors. Materials and Methods: First, the supernatant extract and lysate were prepared from the native strain of Saccharomyces cerevisiae and turned into dry powder. Then, supernatant and lysate extracts were admixed with Pseudomonas aeruginosa strain PAO1 and strain M 8821, respectively, and biofilm in the strain PAO1 and alginate in strain 8821 M by Colorimetric method is measured by reading Optical Density(OD) also mention to the wave length. Supernatant with MIC concentration of 1/2 in both experiments and all concentrations of lysates in biofilm test and the highest concentration of lysates in alginate test were used. Results: Supernatant of Saccharomyces cerevisiae at a concentration of 1 / 2MIC (0.512 mg / ml) with P <0.05 significantly reduced the production of alginate in Pseudomonas aeruginosa 8821 M strain, but did not affect biofilm in Pseudomonas aeruginosa PAO1 strain respectively, with all concentrations and the highest concentration (8.192 mg / ml) with P <0.05 significantly reduced the production of biofilms in Pseudomonas aeruginosa strain PAO1 and alginate in Pseudomonas aeruginosa 8821 M strain. Conclusion: Saccharomyces cerevisiae is one of the options that probiotic studies can examine its effects on virulence pathogens, but requires the ultimate goal of producing probiotic drugs.

Keywords

• Pseudomonas aeruginosa
• Saccharomyces cerevisiae
• alginate
• biofilm