Transplant International (May 2024)
Machine Perfusion Enables 24-h Preservation of Vascularized Composite Allografts in a Swine Model of Allotransplantation
- Marion Goutard,
- Marion Goutard,
- Marion Goutard,
- Pierre Tawa,
- Pierre Tawa,
- Pierre Tawa,
- Yanis Berkane,
- Yanis Berkane,
- Yanis Berkane,
- Yanis Berkane,
- Yanis Berkane,
- Alec R. Andrews,
- Alec R. Andrews,
- Alec R. Andrews,
- Casie A. Pendexter,
- Casie A. Pendexter,
- Casie A. Pendexter,
- Reinier J. de Vries,
- Reinier J. de Vries,
- Reinier J. de Vries,
- Reinier J. de Vries,
- Victor Pozzo,
- Victor Pozzo,
- Victor Pozzo,
- Golda Romano,
- Golda Romano,
- Golda Romano,
- Hyshem H. Lancia,
- Hyshem H. Lancia,
- Hyshem H. Lancia,
- Irina Filz von Reiterdank,
- Irina Filz von Reiterdank,
- Irina Filz von Reiterdank,
- Irina Filz von Reiterdank,
- Irina Filz von Reiterdank,
- Nicolas Bertheuil,
- Nicolas Bertheuil,
- Ivy A. Rosales,
- Ivy A. Rosales,
- Ira Doressa Anne L. How,
- Ira Doressa Anne L. How,
- Mark A. Randolph,
- Mark A. Randolph,
- Mark A. Randolph,
- Alexandre G. Lellouch,
- Alexandre G. Lellouch,
- Alexandre G. Lellouch,
- Curtis L. Cetrulo,
- Curtis L. Cetrulo,
- Curtis L. Cetrulo,
- Korkut Uygun,
- Korkut Uygun,
- Korkut Uygun
Affiliations
- Marion Goutard
- Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston, MA, United States
- Marion Goutard
- Harvard Medical School, Boston, MA, United States
- Marion Goutard
- Shriners Children’s Boston, Boston, MA, United States
- Pierre Tawa
- Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston, MA, United States
- Pierre Tawa
- Harvard Medical School, Boston, MA, United States
- Pierre Tawa
- Shriners Children’s Boston, Boston, MA, United States
- Yanis Berkane
- Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston, MA, United States
- Yanis Berkane
- Harvard Medical School, Boston, MA, United States
- Yanis Berkane
- Shriners Children’s Boston, Boston, MA, United States
- Yanis Berkane
- Suivi Immunologique des Thérapeutiques Innovantes Laboratory, INSERM U1236, University of Rennes 1, Rennes, France
- Yanis Berkane
- Department of Plastic, Reconstructive, and Aesthetic Surgery, Centre Hospitalier Universitaire de Rennes, Université de Rennes 1, Rennes, France
- Alec R. Andrews
- Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston, MA, United States
- Alec R. Andrews
- Harvard Medical School, Boston, MA, United States
- Alec R. Andrews
- Shriners Children’s Boston, Boston, MA, United States
- Casie A. Pendexter
- Harvard Medical School, Boston, MA, United States
- Casie A. Pendexter
- Shriners Children’s Boston, Boston, MA, United States
- Casie A. Pendexter
- Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, Boston, MA, United States
- Reinier J. de Vries
- Harvard Medical School, Boston, MA, United States
- Reinier J. de Vries
- Shriners Children’s Boston, Boston, MA, United States
- Reinier J. de Vries
- Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, Boston, MA, United States
- Reinier J. de Vries
- Department of Surgery, Amsterdam University Medical Centers—Location AMC, University of Amsterdam, Amsterdam, Netherlands
- Victor Pozzo
- Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston, MA, United States
- Victor Pozzo
- Harvard Medical School, Boston, MA, United States
- Victor Pozzo
- Shriners Children’s Boston, Boston, MA, United States
- Golda Romano
- Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston, MA, United States
- Golda Romano
- Harvard Medical School, Boston, MA, United States
- Golda Romano
- Shriners Children’s Boston, Boston, MA, United States
- Hyshem H. Lancia
- Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston, MA, United States
- Hyshem H. Lancia
- Harvard Medical School, Boston, MA, United States
- Hyshem H. Lancia
- Shriners Children’s Boston, Boston, MA, United States
- Irina Filz von Reiterdank
- Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston, MA, United States
- Irina Filz von Reiterdank
- Harvard Medical School, Boston, MA, United States
- Irina Filz von Reiterdank
- Shriners Children’s Boston, Boston, MA, United States
- Irina Filz von Reiterdank
- Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, Boston, MA, United States
- Irina Filz von Reiterdank
- University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands
- Nicolas Bertheuil
- Suivi Immunologique des Thérapeutiques Innovantes Laboratory, INSERM U1236, University of Rennes 1, Rennes, France
- Nicolas Bertheuil
- Department of Plastic, Reconstructive, and Aesthetic Surgery, Centre Hospitalier Universitaire de Rennes, Université de Rennes 1, Rennes, France
- Ivy A. Rosales
- Immunopathology Research Laboratory, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, United States
- Ivy A. Rosales
- 0Department of Pathology, Harvard Medical School, Boston, MA, United States
- Ira Doressa Anne L. How
- Immunopathology Research Laboratory, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, United States
- Ira Doressa Anne L. How
- 0Department of Pathology, Harvard Medical School, Boston, MA, United States
- Mark A. Randolph
- Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston, MA, United States
- Mark A. Randolph
- Harvard Medical School, Boston, MA, United States
- Mark A. Randolph
- Shriners Children’s Boston, Boston, MA, United States
- Alexandre G. Lellouch
- Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston, MA, United States
- Alexandre G. Lellouch
- Harvard Medical School, Boston, MA, United States
- Alexandre G. Lellouch
- Shriners Children’s Boston, Boston, MA, United States
- Curtis L. Cetrulo
- Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston, MA, United States
- Curtis L. Cetrulo
- Harvard Medical School, Boston, MA, United States
- Curtis L. Cetrulo
- Shriners Children’s Boston, Boston, MA, United States
- Korkut Uygun
- Harvard Medical School, Boston, MA, United States
- Korkut Uygun
- Shriners Children’s Boston, Boston, MA, United States
- Korkut Uygun
- Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, Boston, MA, United States
- DOI
- https://doi.org/10.3389/ti.2024.12338
- Journal volume & issue
-
Vol. 37
Abstract
The current gold standard for preserving vascularized composite allografts (VCA) is 4°C static cold storage (SCS), albeit muscle vulnerability to ischemia can be described as early as after 2 h of SCS. Alternatively, machine perfusion (MP) is growing in the world of organ preservation. Herein, we investigated the outcomes of oxygenated acellular subnormothermic machine perfusion (SNMP) for 24-h VCA preservation before allotransplantation in a swine model. Six partial hindlimbs were procured on adult pigs and preserved ex vivo for 24 h with either SNMP (n = 3) or SCS (n = 3) before heterotopic allotransplantation. Recipient animals received immunosuppression and were followed up for 14 days. Clinical monitoring was carried out twice daily, and graft biopsies and blood samples were regularly collected. Two blinded pathologists assessed skin and muscle samples. Overall survival was higher in the SNMP group. Early euthanasia of 2 animals in the SCS group was linked to significant graft degeneration. Analyses of the grafts showed massive muscle degeneration in the SCS group and a normal aspect in the SNMP group 2 weeks after allotransplantation. Therefore, this 24-h SNMP protocol using a modified Steen solution generated better clinical and histological outcomes in allotransplantation when compared to time-matched SCS.
Keywords
- vascularized composite allotransplantation
- machine perfusion
- organ preservation
- ischemia-reperfusion injuries
- VCA
