Vasodilation promoted by (E,E)-farnesol involving ion channels in human umbilical arteries
Paulo Ricardo Batista,
Andressa de Alencar Silva,
Carla Mikevely de Sena Bastos,
Renata Evaristo Rodrigues da Silva,
Gabriela Lucena Calixto,
Luís Pereira de Morais,
Gyllyandeson de Araújo Delmondes,
Marta Regina Kerntopf,
Irwin Rose Alencar de Menezes,
Roseli Barbosa
Affiliations
Paulo Ricardo Batista
Biological Chemistry Department, Pimenta Campus, Regional University of Cariri, Crato, 63105-000, Ceará, Brazil; Biological Sciences Department, Physiopharmacology of Excitable Cells Laboratory, Pimenta Campus, Regional University of Cariri, Crato, 63105-000, Ceará, Brazil
Andressa de Alencar Silva
Graduate Program in Physiological Sciences, Higher Institute of Biomedical Sciences, State University of Ceará, Fortaleza, 60714-903, Ceará, Brazil; Biological Sciences Department, Physiopharmacology of Excitable Cells Laboratory, Pimenta Campus, Regional University of Cariri, Crato, 63105-000, Ceará, Brazil
Carla Mikevely de Sena Bastos
Biological Chemistry Department, Pimenta Campus, Regional University of Cariri, Crato, 63105-000, Ceará, Brazil; Biological Sciences Department, Physiopharmacology of Excitable Cells Laboratory, Pimenta Campus, Regional University of Cariri, Crato, 63105-000, Ceará, Brazil
Renata Evaristo Rodrigues da Silva
Biological Sciences Department, Physiopharmacology of Excitable Cells Laboratory, Pimenta Campus, Regional University of Cariri, Crato, 63105-000, Ceará, Brazil
Gabriela Lucena Calixto
Biological Sciences Department, Physiopharmacology of Excitable Cells Laboratory, Pimenta Campus, Regional University of Cariri, Crato, 63105-000, Ceará, Brazil
Luís Pereira de Morais
Biological Sciences Department, Physiopharmacology of Excitable Cells Laboratory, Pimenta Campus, Regional University of Cariri, Crato, 63105-000, Ceará, Brazil; Biotechnology By the Northeastern Biotechnology Network (RENORBIO), State University of Ceará, Fortaleza, 60714-903, Ceará, Brazil
Gyllyandeson de Araújo Delmondes
Nursing Collegiate, Petrolina Campus, Federal University of the San Francisco Vale, Petrolina, 56304-205, Pernambuco, Brazil
Marta Regina Kerntopf
Biological Chemistry Department, Pimenta Campus, Regional University of Cariri, Crato, 63105-000, Ceará, Brazil
Irwin Rose Alencar de Menezes
Biological Chemistry Department, Pimenta Campus, Regional University of Cariri, Crato, 63105-000, Ceará, Brazil; Corresponding author. Biological Chemistry Department, Pimenta Campus, Regional University of Cariri, Rua Coronel Antônio Luiz 1161, Crato, 63105-000, Ceará, Brazil.
Roseli Barbosa
Biological Chemistry Department, Pimenta Campus, Regional University of Cariri, Crato, 63105-000, Ceará, Brazil; Biological Sciences Department, Physiopharmacology of Excitable Cells Laboratory, Pimenta Campus, Regional University of Cariri, Crato, 63105-000, Ceará, Brazil; Corresponding author. Biological Chemistry Department, Pimenta Campus, Regional University of Cariri, Rua Coronel Antônio Luiz 1161, Crato, 63105-000, Ceará, Brazil.
Background: (E,E)-farnesol is a sesquiterpene alcohol derived from plants and animals that exhibits pharmacological properties in the cardiovascular system. However, its effects on human umbilical vessels remain unknown. Purpose: Thus, this study aims to characterize the vasodilatory effect of (E,E)-farnesol in human umbilical arteries (HUA). Study design: The tissue is obtained from pregnant women over 18 years of age, normotensive, and without prepartum complications. After collected, the tissue was segmented and dissected to remove Wharton's jelly and obtain the umbilical arteries segments. Methods: HUA segments were isolated and sectioned into rings that were subjected to isometric tension recordings in an organ bath. Results: (E,E)-farnesol (1 μmol/L to 1 mmol/L) promoted vasodilatory effect in HUA preparations, affecting basal tone, and inhibiting the electromechanical coupling induced by KCl 60 mmol/L with greater potency (EC50 225.3 μmol/L) than the pharmacomechanical coupling induced by 5-HT 10 μmol/L (EC50 363.5 μmol/L). In the absence of extracellular calcium, pharmacomechanical coupling was also abolished, and contractions induced by CaCl2 or BaCl2 were attenuated by (E,E)-farnesol indicating a possible direct inhibition of L-type VOCC as a mechanism of the vasodilatory effect. The vasodilator efficacy of (E,E)-farnesol on reduction of vasocontraction induced by the presence of tetraethylammonium (1 or 10 mmol/L), 4-aminopyridine (1 mmol/L) and glibenclamide (10 μmol/L) suggesting a possible influence of different potassium channels (BKCa, KV and KATP). Conclusion: These results suggest that (E,E)-farnesol may be a promising pharmacological candidate for obstetric hypertensive disorders.
