Effect of Extender, Storage Time and Temperature on Kinetic Parameters (CASA) on Bull Semen Samples
Aitor Fernandez-Novo,
Sergio Santos-Lopez,
Clara Barrajon-Masa,
Patricia Mozas,
Eduardo de Mercado,
Elisa Caceres,
Aizic Garrafa,
Juan V. Gonzalez-Martin,
Natividad Perez-Villalobos,
Agustín Oliet,
Susana Astiz,
Sonia S. Perez-Garnelo
Affiliations
Aitor Fernandez-Novo
Department of Veterinary Medicine, School of Biomedical and Health Sciences, Universidad Europea de Madrid, Madrid, C/Tajo s/n, Villaviciosa de Odón, 28670 Madrid, Spain
Sergio Santos-Lopez
Animal Production Department, Veterinary Faculty, Complutense Univeristy of Madrid, Puerta de Hierro Avenue s/n, 28040 Madrid, Spain
Clara Barrajon-Masa
National Centre of Selection and Animal Reproduction (CENSYRA) of Colmenar Viejo. Ctra, Colmenar Viejo–Guadalix de la Sierra km 1, Guadalix de la Sierra, 28794 Madrid, Spain
Patricia Mozas
National Centre of Selection and Animal Reproduction (CENSYRA) of Colmenar Viejo. Ctra, Colmenar Viejo–Guadalix de la Sierra km 1, Guadalix de la Sierra, 28794 Madrid, Spain
Eduardo de Mercado
Animal Reproduction Department of National Institute of Agronomic Research (INIA), Puerta de Hierro Avenue s/n, 28040 Madrid, Spain
Elisa Caceres
Medicine and Surgery Animal Department, Veterinary Faculty, Complutense University of Madrid, Puerta de Hierro Avenue s/n, 28040 Madrid, Spain
Aizic Garrafa
Medicine and Surgery Animal Department, Veterinary Faculty, Complutense University of Madrid, Puerta de Hierro Avenue s/n, 28040 Madrid, Spain
Juan V. Gonzalez-Martin
Medicine and Surgery Animal Department, Veterinary Faculty, Complutense University of Madrid, Puerta de Hierro Avenue s/n, 28040 Madrid, Spain
Natividad Perez-Villalobos
Department of Veterinary Medicine, School of Biomedical and Health Sciences, Universidad Europea de Madrid, Madrid, C/Tajo s/n, Villaviciosa de Odón, 28670 Madrid, Spain
Agustín Oliet
National Centre of Selection and Animal Reproduction (CENSYRA) of Colmenar Viejo. Ctra, Colmenar Viejo–Guadalix de la Sierra km 1, Guadalix de la Sierra, 28794 Madrid, Spain
Susana Astiz
Animal Reproduction Department of National Institute of Agronomic Research (INIA), Puerta de Hierro Avenue s/n, 28040 Madrid, Spain
Sonia S. Perez-Garnelo
Animal Reproduction Department of National Institute of Agronomic Research (INIA), Puerta de Hierro Avenue s/n, 28040 Madrid, Spain
CASA kinetic parameters are often evaluated in a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, in 25 commercial bulls electroejaculated in the field, the impact of time until evaluation (0–2 h, 4–6 h, and 24 h post-ejaculation), holding temperature (5 °C vs. room temperature), and extender (AndroMed®, BIOXcell® or INRA96®) on CASA kinetic parameters. Total and progressive motility, VCL, VAP, VCL, ALH, BCF, STR, LIN, and WOB were assessed. CASA kinetic parameters were preserved for up to 4–6 h post-ejaculation, except for AndroMed®. Regardless of extender or temperature, motility decreased from 4–6 h up to 24 h, with the best values obtained with BIOXcell® at 5 °C. Our results suggest that BIOXcell® can preserve sperm motility for up to 6 h, either at 5 °C or room temperature, and also INRA96® at room temperature, with motility assessments and the percentage of the most rapid sperms being the lowest with INRA96® at 5 °C. The kinetic parameters decreased when analyses were performed at 24 h. Therefore, we suggest evaluating seminal quality as soon as possible, before 6 h after collection. These results help to fix adequate protocols for the short-term storage and shipment of bovine semen collected under field conditions.
