Responses of maize germination, root morphology and leaf trait to characteristics of lead pollution: a case study

Abstract

Abstract On base of the content of Pb in the soil under different land use patterns in Lanping Lead-zinc mining area, Yunnan in southwest China, the root morphology and leaf traits of maize in different concentration Pb (20, 40, 60, 80, 100, 150, 200, 500, 1000, 2000, 3000 mg/L) were analyzed. The results showed that maize germination rate, germination vigor and growth index decreased with the increase of Pb concentration. The root length, surface area of maize increased by 0.21%–81.58%, 8.99%–73.43%, 1.50%–77.37%, respectively, under 20–500 mg/L Pb concentration. However, these parameters under 1000–3000 mg/L Pb concentration decreased by 37.86%–553.54%, 44.99%–766.16%, 55.99%–92.81%, respectively, and these lowest value appeared in 3000 mg/L Pb treatment. The root volume of maize increased by 4.57%–89.25% in 20–80 mg/L Pb concentration, and it decreased with the increase of Pb concentration when the Pb concentration was higher than 80 mg/L and decreased by 94.13% in 3000 mg/L Pb. The root surface area and length of 0.50–1.00 diameter class were higher than those of other diameter classes, and these value of maize under 500 mg/L Pb were higher than those of other concentrations. The length and perimeter of maize leaves with the highest value of 220.36 and 962.68 mm, respectively appeared in 60 mg/L Pb treatment. The leaf width and area of maize with the highest value of 15.68 mm and 2448.31 mm2, respectively, appeared in 40 mg/L Pb treatment, which indicated that the leaf traits of maize were promoted by low concentration Pb and inhibited by high concentration Pb.

Keywords

• Pb stress
• Root morphology
• Leaf traits
• Seed germination