Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level

Lucia Gardini; Sarah M. Heissler; Claudia Arbore; Yi Yang; James R. Sellers; Francesco S. Pavone; Marco Capitanio

doi:10.1038/s41467-018-05251-z

Nature Communications (Jul 2018)

Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level

Lucia Gardini,
Sarah M. Heissler,
Claudia Arbore,
Yi Yang,
James R. Sellers,
Francesco S. Pavone,
Marco Capitanio

Affiliations

Lucia Gardini: LENS—European Laboratory for Non-linear Spectroscopy, University of Florence
Sarah M. Heissler: Laboratory of Molecular Physiology, National Heart, Lung, and Blood Institute, National Institutes of Health
Claudia Arbore: LENS—European Laboratory for Non-linear Spectroscopy, University of Florence
Yi Yang: Laboratory of Molecular Physiology, National Heart, Lung, and Blood Institute, National Institutes of Health
James R. Sellers: Laboratory of Molecular Physiology, National Heart, Lung, and Blood Institute, National Institutes of Health
Francesco S. Pavone: LENS—European Laboratory for Non-linear Spectroscopy, University of Florence
Marco Capitanio: LENS—European Laboratory for Non-linear Spectroscopy, University of Florence

DOI: https://doi.org/10.1038/s41467-018-05251-z
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 12

Abstract

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Myosin-5B is an actin-based motor important for endosome recycling, but the molecular mechanism underlying its motility remains unknown. Here authors use single molecule imaging and high-speed laser tweezers to dissect the mechanoenzymatic properties of myosin-5B, which shows processive motility with peculiar mechanosensitivity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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