Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation

Mitchell Henry Wright; Joseph Adelskov; Anthony Carlson Greene

doi:10.1128/jmbe.v18i2.1348

Journal of Microbiology & Biology Education (Sep 2017)

Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation

Mitchell Henry Wright,
Joseph Adelskov,
Anthony Carlson Greene

Affiliations

Mitchell Henry Wright: Division of Environmental and Biomolecular Systems, Institute of Environmental Health, Oregon Health & Science University, Portland, OR 97239-3098
Joseph Adelskov: School of Natural Sciences, Griffith University, Nathan Campus, Queensland, Australia
Anthony Carlson Greene: School of Natural Sciences, Griffith University, Nathan Campus, Queensland, Australia

DOI: https://doi.org/10.1128/jmbe.v18i2.1348
Journal volume & issue: Vol. 18, no. 2

Abstract

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Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and discuss how this can be incorporated into microbiology laboratory courses for biology majors.

Published in Journal of Microbiology & Biology Education

ISSN: 1935-7877 (Print); 1935-7885 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Education: Special aspects of education; Science: Biology (General)
Website: https://journals.asm.org/journal/jmbe

About the journal