DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle

Andrey Mikheikin; Anita Olsen; Kevin Leslie; Freddie Russell-Pavier; Andrew Yacoot; Loren Picco; Oliver Payton; Amir Toor; Alden Chesney; James K. Gimzewski; Bud Mishra; Jason Reed

doi:10.1038/s41467-017-01891-9

Nature Communications (Nov 2017)

DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle

Andrey Mikheikin,
Anita Olsen,
Kevin Leslie,
Freddie Russell-Pavier,
Andrew Yacoot,
Loren Picco,
Oliver Payton,
Amir Toor,
Alden Chesney,
James K. Gimzewski,
Bud Mishra,
Jason Reed

Affiliations

Andrey Mikheikin: Department of Physics, Virginia Commonwealth University
Anita Olsen: Department of Physics, Virginia Commonwealth University
Kevin Leslie: Department of Physics, Virginia Commonwealth University
Freddie Russell-Pavier: National Physical Laboratory
Andrew Yacoot: National Physical Laboratory
Loren Picco: Interface Analysis Centre, H. H. Wills Physics Laboratory
Oliver Payton: Interface Analysis Centre, H. H. Wills Physics Laboratory
Amir Toor: Department of Internal Medicine, VCU School of Medicine
Alden Chesney: VCU Massey Cancer Center
James K. Gimzewski: Department of Chemistry and Biochemistry, UCLA
Bud Mishra: Departments of Computer Science and Mathematics, Courant Institute of Mathematical Sciences, New York University
Jason Reed: Department of Physics, Virginia Commonwealth University

DOI: https://doi.org/10.1038/s41467-017-01891-9
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 9

Abstract

Read online

Physical mapping of DNA can be used to detect structural variants and for whole-genome haplotype assembly. Here, the authors use CRISPR-Cas9 and high-speed atomic force microscopy to ‘nanomap’ single molecules of DNA.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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