Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta umbla Gill and Liver Tissues and Inhibition Effects of Some Metal Ions on Enzyme Activity

Abstract

In this study, the in vitro effects of some metal ions (Ag+, Cd2+, Cu2+, Fe2+, Ni2+ and Pb2+) on freshwater fish Capoeta umbla liver and gill glucose 6-phosphate dehydrogenase (G6PD) have been investigated. For this purpose, C. umbla liver and gill G6PD enzymes were purified, with a specific activity of 31.52 and 22.83 EU/mg protein, 3353.2 and 736.5 fold in a yield of 19.28% and 23.15%, respectively. In order to control the enzyme purification SDS-PAGE showed a single band for the enzyme. In addition, in vitro effects of metal ions on enzyme activity were researched. As a result, Ag+, Cd2+, Cu2+, Ni2+ and Pb2+ inhibited fish liver G6PD; Ag+, Cd2+, Fe2+, Ni2+ and Pb2+ inhibited fish gill G6PD. Besides, it was found that the most effective inhibitor of G6PD enzyme within metal ions used was Ag+. Our results also demonstrate that these metals might be dangerous at low micromolar concentrations for fish G6PD enzyme.

Keywords

• glucose 6-phosphate dehydrogenase
• capoeta umbla
• enzyme purification
• inhibition
• metal ions
• liver