Communications Biology (Mar 2021)
Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics
- Chia-Feng Tsai,
- Pengfei Zhang,
- David Scholten,
- Kendall Martin,
- Yi-Ting Wang,
- Rui Zhao,
- William B. Chrisler,
- Dhwani B. Patel,
- Maowei Dou,
- Yuzhi Jia,
- Carolina Reduzzi,
- Xia Liu,
- Ronald J. Moore,
- Kristin E. Burnum-Johnson,
- Miao-Hsia Lin,
- Chuan-Chih Hsu,
- Jon M. Jacobs,
- Jacob Kagan,
- Sudhir Srivastava,
- Karin D. Rodland,
- H. Steven Wiley,
- Wei-Jun Qian,
- Richard D. Smith,
- Ying Zhu,
- Massimo Cristofanilli,
- Tao Liu,
- Huiping Liu,
- Tujin Shi
Affiliations
- Chia-Feng Tsai
- Biological Sciences Division, Pacific Northwest National Laboratory
- Pengfei Zhang
- Biological Sciences Division, Pacific Northwest National Laboratory
- David Scholten
- Department of Pharmacology, Feinberg School of Medicine, Northwestern University
- Kendall Martin
- Biological Sciences Division, Pacific Northwest National Laboratory
- Yi-Ting Wang
- Biological Sciences Division, Pacific Northwest National Laboratory
- Rui Zhao
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- William B. Chrisler
- Biological Sciences Division, Pacific Northwest National Laboratory
- Dhwani B. Patel
- Department of Pharmacology, Feinberg School of Medicine, Northwestern University
- Maowei Dou
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- Yuzhi Jia
- Department of Pharmacology, Feinberg School of Medicine, Northwestern University
- Carolina Reduzzi
- Division of Hematology and Oncology, Department of Medicine, Feinberg School of Medicine, Northwestern University
- Xia Liu
- Department of Pharmacology, Feinberg School of Medicine, Northwestern University
- Ronald J. Moore
- Biological Sciences Division, Pacific Northwest National Laboratory
- Kristin E. Burnum-Johnson
- Biological Sciences Division, Pacific Northwest National Laboratory
- Miao-Hsia Lin
- Graduate Institute of Microbiology, College of Medicine, National Taiwan University
- Chuan-Chih Hsu
- Institute of Plant and Microbial Biology, Academia Sinica
- Jon M. Jacobs
- Biological Sciences Division, Pacific Northwest National Laboratory
- Jacob Kagan
- Cancer Biomarkers Research Group, Division of Cancer Prevention, National Cancer Institute
- Sudhir Srivastava
- Cancer Biomarkers Research Group, Division of Cancer Prevention, National Cancer Institute
- Karin D. Rodland
- Biological Sciences Division, Pacific Northwest National Laboratory
- H. Steven Wiley
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- Wei-Jun Qian
- Biological Sciences Division, Pacific Northwest National Laboratory
- Richard D. Smith
- Biological Sciences Division, Pacific Northwest National Laboratory
- Ying Zhu
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- Massimo Cristofanilli
- Division of Hematology and Oncology, Department of Medicine, Feinberg School of Medicine, Northwestern University
- Tao Liu
- Biological Sciences Division, Pacific Northwest National Laboratory
- Huiping Liu
- Department of Pharmacology, Feinberg School of Medicine, Northwestern University
- Tujin Shi
- Biological Sciences Division, Pacific Northwest National Laboratory
- DOI
- https://doi.org/10.1038/s42003-021-01797-9
- Journal volume & issue
-
Vol. 4,
no. 1
pp. 1 – 12
Abstract
Tsai, Zhang, Scholten et al. develop a surfactant- assisted one-pot sample preparation coupled with mass spectrometry method (SOP-MS) for label-free global single-cell proteomics. This method allows researchers to measure hundreds of proteins from single human cells, suggesting its utility for quantitative single-cell proteomics.
