Stem Cell Research (Jul 2016)

Establishment of induced pluripotent stem cell (iPSC) line from an 84-year old patient with late onset Alzheimer's disease (LOAD)

  • Zsuzsanna Táncos,
  • Eszter Varga,
  • Eszter Kovács,
  • András Dinnyés,
  • Julianna Kobolák

DOI
https://doi.org/10.1016/j.scr.2016.05.016
Journal volume & issue
Vol. 17, no. 1
pp. 75 – 77

Abstract

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Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterised 84-year old male with late onset Alzheimer's disease (LOAD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously towards the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to further study the pathomechanism of sporadic AD, to identify early biomarkers and also for drug testing and gene therapy studies.