Communications Biology (Jun 2021)
Genome-wide CRISPR screen identifies protein pathways modulating tau protein levels in neurons
- Carlos G. Sanchez,
- Christopher M. Acker,
- Audrey Gray,
- Malini Varadarajan,
- Cheng Song,
- Nadire R. Cochran,
- Steven Paula,
- Alicia Lindeman,
- Shaojian An,
- Gregory McAllister,
- John Alford,
- John Reece-Hoyes,
- Carsten Russ,
- Lucas Craig,
- Ketthsy Capre,
- Christian Doherty,
- Gregory R. Hoffman,
- Sarah J. Luchansky,
- Manuela Polydoro,
- Ricardo Dolmetsch,
- Fiona Elwood
Affiliations
- Carlos G. Sanchez
- Department of Neuroscience, Novartis Institutes for BioMedical Research
- Christopher M. Acker
- Department of Neuroscience, Novartis Institutes for BioMedical Research
- Audrey Gray
- Department of Neuroscience, Novartis Institutes for BioMedical Research
- Malini Varadarajan
- Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research
- Cheng Song
- Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research
- Nadire R. Cochran
- Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research
- Steven Paula
- Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research
- Alicia Lindeman
- Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research
- Shaojian An
- Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research
- Gregory McAllister
- Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research
- John Alford
- Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research
- John Reece-Hoyes
- Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research
- Carsten Russ
- Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research
- Lucas Craig
- Department of Neuroscience, Novartis Institutes for BioMedical Research
- Ketthsy Capre
- Department of Neuroscience, Novartis Institutes for BioMedical Research
- Christian Doherty
- Department of Neuroscience, Novartis Institutes for BioMedical Research
- Gregory R. Hoffman
- Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research
- Sarah J. Luchansky
- Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research
- Manuela Polydoro
- Department of Neuroscience, Novartis Institutes for BioMedical Research
- Ricardo Dolmetsch
- Department of Neuroscience, Novartis Institutes for BioMedical Research
- Fiona Elwood
- Department of Neuroscience, Novartis Institutes for BioMedical Research
- DOI
- https://doi.org/10.1038/s42003-021-02272-1
- Journal volume & issue
-
Vol. 4,
no. 1
pp. 1 – 14
Abstract
Using an unbiased genome-wide CRISPR screen approach, Sanchez et al. identified modulators of endogenous tau protein. This study suggests that chromatin modifiers, neddylation, ubiquitination, and the mTOR pathways regulate overall levels of tau protein in neurons, which could help in future identification of therapeutics for neurodegenerative diseases.
