PURIFICATION AND QUANTIFICATION OF FIBRONECTIN TYPE II PROTEINS IN MALABARI BUCK SEMINAL PLASMA

Abstract

The study was conducted to purify and quantify Fn2 type (Fibronectin type II) proteins of Malabari buck seminal plasma. Quantification of whole seminal plasma protein did not reveal any significant difference between bucks with good and poor semen freezability, while significant difference was observed in the quantity of Fn2 proteins in the two groups of bucks. SDS-PAGE analysis revealed four gelatin binding proteins with apparent molecular weight 14kDa, 15kDa, 20kDa and 22kDa.

Keywords

• fn2 type proteins
• buck seminal plasma
• affinity chromatography