Nature Communications (Aug 2022)
Generation of a CRISPR activation mouse that enables modelling of aggressive lymphoma and interrogation of venetoclax resistance
- Yexuan Deng,
- Sarah T. Diepstraten,
- Margaret A. Potts,
- Göknur Giner,
- Stephanie Trezise,
- Ashley P. Ng,
- Gerry Healey,
- Serena R. Kane,
- Amali Cooray,
- Kira Behrens,
- Amy Heidersbach,
- Andrew J. Kueh,
- Martin Pal,
- Stephen Wilcox,
- Lin Tai,
- Warren S. Alexander,
- Jane E. Visvader,
- Stephen L. Nutt,
- Andreas Strasser,
- Benjamin Haley,
- Quan Zhao,
- Gemma L. Kelly,
- Marco J. Herold
Affiliations
- Yexuan Deng
- The State Key Laboratory of Pharmaceutical Biotechnology, Department of Hepatobiliary Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, School of Life Sciences, Nanjing University
- Sarah T. Diepstraten
- The Walter and Eliza Hall Institute of Medical Research
- Margaret A. Potts
- The Walter and Eliza Hall Institute of Medical Research
- Göknur Giner
- The Walter and Eliza Hall Institute of Medical Research
- Stephanie Trezise
- The Walter and Eliza Hall Institute of Medical Research
- Ashley P. Ng
- The Walter and Eliza Hall Institute of Medical Research
- Gerry Healey
- The Walter and Eliza Hall Institute of Medical Research
- Serena R. Kane
- The Walter and Eliza Hall Institute of Medical Research
- Amali Cooray
- The Walter and Eliza Hall Institute of Medical Research
- Kira Behrens
- The Walter and Eliza Hall Institute of Medical Research
- Amy Heidersbach
- School of Dentistry and Medical Sciences, Charles Sturt University
- Andrew J. Kueh
- The Walter and Eliza Hall Institute of Medical Research
- Martin Pal
- The Walter and Eliza Hall Institute of Medical Research
- Stephen Wilcox
- The Walter and Eliza Hall Institute of Medical Research
- Lin Tai
- The Walter and Eliza Hall Institute of Medical Research
- Warren S. Alexander
- The Walter and Eliza Hall Institute of Medical Research
- Jane E. Visvader
- The Walter and Eliza Hall Institute of Medical Research
- Stephen L. Nutt
- The Walter and Eliza Hall Institute of Medical Research
- Andreas Strasser
- The Walter and Eliza Hall Institute of Medical Research
- Benjamin Haley
- Department of Molecular Biology, Genentech, Inc.
- Quan Zhao
- The State Key Laboratory of Pharmaceutical Biotechnology, Department of Hepatobiliary Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, School of Life Sciences, Nanjing University
- Gemma L. Kelly
- The Walter and Eliza Hall Institute of Medical Research
- Marco J. Herold
- The Walter and Eliza Hall Institute of Medical Research
- DOI
- https://doi.org/10.1038/s41467-022-32485-9
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 17
Abstract
Modelling of aggressive lymphomas, such as double hit lymphoma, has been challenging. Here the authors engineer a CRISPR activation mouse to enable the generation of these aggressive lymphomas and identify the pro-survival BCL-2 protein A1 as a venetoclax resistance factor.
