Effects of cryo-EM cooling on structural ensembles

Lars V. Bock; Helmut Grubmüller

doi:10.1038/s41467-022-29332-2

Nature Communications (Mar 2022)

Effects of cryo-EM cooling on structural ensembles

Lars V. Bock,
Helmut Grubmüller

Affiliations

Lars V. Bock: Theoretical and Computational Biophysics Department, Max Planck Institute for Multidisciplinary Sciences
Helmut Grubmüller: Theoretical and Computational Biophysics Department, Max Planck Institute for Multidisciplinary Sciences

DOI: https://doi.org/10.1038/s41467-022-29332-2
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

Read online

The rapid temperature drop during plunge-freezing affects the structural ensembles obtained by cryo-EM. To quantify the extent of perturbation, Bock and Grubmüller combined continuum calculations, MD simulations, and kinetic models.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal