Fluorescence microscopy data on expression of Paired Box Transcription Factor 7 in skeletal muscle of APOBEC2 knockout mice
Hideaki Ohtsubo,
Yusuke Sato,
Yuji Matsuyoshi,
Takahiro Suzuki,
Wataru Mizunoya,
Mako Nakamura,
Ryuichi Tatsumi,
Yoshihide Ikeuchi
Affiliations
Hideaki Ohtsubo
Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan
Yusuke Sato
Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan; Department of Bio-Productive Science, Utsunomiya University, Utsunomiya, Tochigi 321-8505, Japan
Yuji Matsuyoshi
Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan
Takahiro Suzuki
Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan; Department of Molecular and Developmental Biology, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan
Wataru Mizunoya
Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan
Mako Nakamura
Graduate School of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan
Ryuichi Tatsumi
Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan; Correspondence to: Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan.
Yoshihide Ikeuchi
Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan; Correspondence to: Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan.
The data presented in this article are related to the research articles entitled “APOBEC2 negatively regulates myoblast differentiation in muscle regeneration” and “Data supporting possible implication of APOBEC2 in self-renewal functions of myogenic stem satellite cells: toward understanding the negative regulation of myoblast differentiation” (Ohtsubo et al., 2017a, 2017b) [1,2]. This article provides in vivo phenotypical data to show that Paired Box Transcription Factor 7 (Pax7)-positive cell number (per myofiber) is significantly lower in APOBEC2 (a member of apoB mRNA editing enzyme, catalytic polypeptide-like family)-knockout muscle than the control wild-type tissue at the same age of 8-wk-old in mice. The emerging results support an essential role for APOBEC2 in the self-renewal functions of myogenic stem satellite cells, namely the re-establishment of quiescent status after activation and proliferation of myoblasts. Keywords: APOBEC2, Myogenic stem satellite cells, Self-renewal, Muscle, Cryo-sections, Immunofluorescence
