Designing Adsorption Centres for Biological Active Molecules on a Silica Surface

Abstract

The chemical interaction between the surface of hydroxylated or aminated silicas and β-cyclodextrin, mono-tosyl-β-cyclodextrin and the bromine derivative of heptakis-[6- O -( p -tosyl-β-cyclodextrin)] has been investigated using IR spectroscopy, thermogravimetric analysis with programmed heating, pH titration, weight adsorption method, elemental analysis and quantitative chemical analysis of the surface compounds. The optimum conditions for chemical grafting of β-cyclodextrins onto the silica surface were established. The formation of supramolecular structures, viz. inclusion complexes between immobilized β-cyclodextrin and the hormone of pineal gland (melatonin), on the surface of highly dispersed silicas of 1:1 composition was demonstrated.