Influence of Endophytic Bacterium, Cellulosimicrobium sp. FRR2 on Plant Growth of Amaranthus campestris L. and Bacterial Survival at Adverse Environmental Conditions

Abstract

The endophytic microorganisms are believed to be an important bio-resource for modern agriculture because of their beneficial effects on plant growth promotion, biocontrol, stress tolerance, and diseases resistance. This study was focused to know the beneficial effect of endophytic bacterium (FRR2) isolated from the roots of Ficus religiosa L. on Amaranthus campestris L. and their tolerance ability against salinity and heavy metals. The strain FRR2 was recognized as Cellulosimicrobium sp. by 16s rRNA sequencing and phylogenetic study. The bacterial isolate FRR2 showed salt (at 150 mM NaCl) and metal (at 150 µM CuSO4 and 100 µM ZnSO4) tolerance ability and significantly higher growth rate of Amaranthus campestris in a green leafy vegetable might be due to the nitrogen fixation, indole acetic acid production, amylase and protease activities. In addition, the endophyte FRR2 application slightly increased the antioxidants activity than their controls. The results of this study revealed that Cellulosimicrobium sp. strain FRR2 would be an effective endophyte to increase the growth of green leafy vegetables.

Keywords

• amaranthus campestris
• cellulosimicrobium sp.
• endophytes
• heavy metal
• salinity