Nanoconjugate Synthesis of <i>Elaeocarpus ganitrus</i> and the Assessment of Its Antimicrobial and Antiproliferative Properties
Arpitha Badarinath Mahajanakatti,
Telugu Seetharam Deepak,
Raghu Ram Achar,
Sushma Pradeep,
Shashanka K Prasad,
Rajeswari Narayanappa,
Deepthi Bhaskar,
Sushravya Shetty,
Govindappa Melappa,
Lavanya Chandramouli,
Sanjukta Mazumdar,
Ekaterina Silina,
Victor Stupin,
Chandrashekar Srinivasa,
Chandan Shivamallu,
Shiva Prasad Kollur
Affiliations
Arpitha Badarinath Mahajanakatti
Department of Biotechnology, Dayananda Sagar College of Engineering, (Affiliated to VTU, Belagavi), Shavige Malleshwara Hills, Bengaluru 560078, Karnataka, India
Telugu Seetharam Deepak
Ramaiah Medical College and Hospitals, New BEL Road, Bengaluru 560054, Karnataka, India
Raghu Ram Achar
Department of Biochemistry, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru 570015, Karnataka, India
Sushma Pradeep
Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru 570015, Karnataka, India
Shashanka K Prasad
Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru 570015, Karnataka, India
Rajeswari Narayanappa
Department of Biotechnology, Dayananda Sagar College of Engineering, (Affiliated to VTU, Belagavi), Shavige Malleshwara Hills, Bengaluru 560078, Karnataka, India
Deepthi Bhaskar
Department of Biotechnology, Dayananda Sagar College of Engineering, (Affiliated to VTU, Belagavi), Shavige Malleshwara Hills, Bengaluru 560078, Karnataka, India
Sushravya Shetty
Department of Biotechnology, Dayananda Sagar College of Engineering, (Affiliated to VTU, Belagavi), Shavige Malleshwara Hills, Bengaluru 560078, Karnataka, India
Govindappa Melappa
Department of Biotechnology, Dayananda Sagar College of Engineering, (Affiliated to VTU, Belagavi), Shavige Malleshwara Hills, Bengaluru 560078, Karnataka, India
Lavanya Chandramouli
Department of Biotechnology, Dayananda Sagar College of Engineering, (Affiliated to VTU, Belagavi), Shavige Malleshwara Hills, Bengaluru 560078, Karnataka, India
Sanjukta Mazumdar
Department of Biotechnology, Dayananda Sagar College of Engineering, (Affiliated to VTU, Belagavi), Shavige Malleshwara Hills, Bengaluru 560078, Karnataka, India
Ekaterina Silina
Department of Human Pathology, Institute of Biodesign and Modeling of Complex Systems, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia
Victor Stupin
Department of Hospital Surgery, N.I. Pirogov Russian National Research Medical University (RNRMU), 117997 Moscow, Russia
Chandrashekar Srinivasa
Department of Biotechnology, Davangere University, Shivagangotri, Davangere 577002, Karnataka, India
Chandan Shivamallu
Department of Biochemistry, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru 570015, Karnataka, India
Shiva Prasad Kollur
School of Agriculture, Geography, Environment, Ocean and Natural Sciences, Laucala Campus, The University of the South Pacific, Suva, Fiji
Cancer is one of the leading causes of death worldwide, accountable for a total of 10 million deaths in the year 2020, according to GLOBOCAN 2020. The advancements in the field of cancer research indicate the need for direction towards the development of new drug candidates that are instrumental in a tumour-specific action. The pool of natural compounds proves to be a promising avenue for the discovery of groundbreaking cancer therapeutics. Elaeocarpus ganitrus (Rudraksha) is known to possess antioxidant properties and after a thorough review of literature, it was speculated to possess significant biomedical potential. Green synthesis of nanoparticles is an environmentally friendly approach intended to eliminate toxic waste and reduce energy consumption. This approach was reported for the synthesis of silver nanoparticles from two different solvent extracts: aqueous and methanolic. These were characterized by biophysical and spectroscopic techniques, namely, UV-Visible Spectroscopy, FTIR, XRD, EDX, DLS, SEM, and GC-MS. The results showed that the nanoconjugates were spherical in geometry. Further, the assessment of antibacterial, antifungal, and antiproliferative activities was conducted which yielded results that were qualitatively positive at the nanoscale. The nanoconjugates were also evaluated for their anticancer properties using a standard MTT Assay. The interactions between the phytochemicals (ligands) and selected cancer receptors were also visualized in silico using the PyRx tool for molecular docking.